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A novel nitric oxide producing probiotic patch and its antimicrobial efficacy: preparation and in vitro analysis

  • Biotechnological Products and Process Engineering
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Abstract

Microbial and fungal infections are a significant consideration in the etiology of all wounds. Numerous antimicrobial and antifungal formulations have been developed with varying degrees of efficacy and stability. Here, we report a nitric oxide producing probiotic adhesive patch device and investigate its antimicrobial and antifungal efficacy in vitro. This probiotic patch utilizes the metabolic activity of immobilized lactic acid bacteria, glucose, and nitrite salts for the production of gaseous nitric oxide (gNO), which is used as an antimicrobial agent against bacterial and fungal pathogens. Results show that application of gNO-producing probiotic patches to cultures of E. coli, S. aureus, P. aeruginosa, MRSA, T. mentagrophytes, and T. rubrum resulted in complete cell death at between 4 and 8 h, and application to cultures of A. baumannii, resulted in fewer than ten colonies detected per milliliter at 6 h. These results demonstrate that a gNO-producing probiotic patch device containing bacteria, glucose, and nitrite salts can produce sufficient levels of gNO over a therapeutically relevant duration to kill common bacterial and fungal wound pathogens in humans.

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Acknowledgements

We acknowledge financial support from the Industrial Research Assistance Program of the National Research Council of Canada (IRAP-NRC) and from Micropharma Limited.

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Authors and Affiliations

  1. Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Physiology, Artificial Cells and Organs Research Centre, Faculty of Medicine McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada

    Mitchell Lawrence Jones, Jorge G. Ganopolsky, Alain Labbé & Satya Prakash

  2. Department of Biomedical Engineering, Faculty of Medicine McGill University, 3775 University Street, Montreal, QC, H3A2B4, Canada

    Mitchell Lawrence Jones & Satya Prakash

  3. Micropharma Limited, 141 avenue du President Kennedy, UQAM, Biological Sciences Building, 5th Floor, Suite 5569, Montreal, QC, H2X3Y7, Canada

    Jorge G. Ganopolsky & Alain Labbé

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  1. Mitchell Lawrence Jones
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  2. Jorge G. Ganopolsky
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  3. Alain Labbé
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  4. Satya Prakash
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Correspondence to Satya Prakash.

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Jones, M.L., Ganopolsky, J.G., Labbé, A. et al. A novel nitric oxide producing probiotic patch and its antimicrobial efficacy: preparation and in vitro analysis. Appl Microbiol Biotechnol 87, 509–516 (2010). https://doi.org/10.1007/s00253-010-2490-x

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  • DOI: https://doi.org/10.1007/s00253-010-2490-x

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