Abstract
This paper reports the effects of changing pH (5–7) and temperature (T, 40–60 °C) on the efficiencies of bacterial hydrolysis of suspended organic matter (SOM) in wastewater from food waste recycling (FWR) and the changes in the bacterial community responsible for this hydrolysis. Maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at pH 5.7 and T = 44.5 °C. Changes in short-chain volatile organic acid profiles and in acidogenic bacterial communities were investigated under these conditions. Propionic and butyric acids concentrations increased rapidly during the first 2 days of incubation. Several band sequences consistent with Clostridium spp. were detected using denaturing gel gradient electrophoresis. Clostridium thermopalmarium and Clostridium novyi seemed to contribute to butyric acid production during the first 1.5 days of acidification of FWR wastewater, and C. thermopalmarium was a major butyric acid producer afterward. C. novyi was an important propionic acid producer. These two species appear to be important contributors to hydrolysis of SOM in the wastewater. Other acidogenic anaerobes, Aeromonas sharmana, Bacillus coagulans, and Pseudomonas plecoglossicida, were also indentified.
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Acknowledgements
This research was supported by the Korea Ministry of Knowledge and Economy (MKE) as the Manpower Development Program for Energy and Resources and the Ministry of Environment (MOE) as the Human Resource Development Project for Waste to Energy. This work was also supported by the Korea Ministry of Education, Science, and Technology (MEST) through the BK-21 program.
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Kim, M.D., Song, M., Jo, M. et al. Growth condition and bacterial community for maximum hydrolysis of suspended organic materials in anaerobic digestion of food waste-recycling wastewater. Appl Microbiol Biotechnol 85, 1611–1618 (2010). https://doi.org/10.1007/s00253-009-2316-x
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DOI: https://doi.org/10.1007/s00253-009-2316-x