Abstract
The biodegradation of toluene was studied in two lab-scale air biofilters operated in parallel, packed respectively with perlite granules (PEG) and polyurethane foam cubes (PUC) and inoculated with the same toluene-degrading fungus. Differences on the material pore size, from micrometres in PEG to millimetres in PUC, were responsible for distinct biomass growth patterns. A compact biofilm was formed around PEG, being the interstitial spaces progressively filled with biomass. Microbial growth concentrated at the core of PUC and the excess of biomass was washed-off, remaining the gas pressure drop comparatively low. Air dispersion in the bed was characterised by tracer studies and modelled as a series of completely stirred tanks (CSTR). The obtained number of CSTR (n) in the PEG packing increased from 33 to 86 along with the applied gas flow (equivalent to empty bed retention times from 48 to 12 s) and with operation time (up to 6 months). In the PUC bed, n varied between 9 and 13, indicating that a stronger and steadier gas dispersion was achieved. Michaelis–Menten half saturation constant (k m) estimates ranged 71–113 mg m−3, depending on the experimental conditions, but such differences were not significant at a 95% confidence interval. The maximum volumetric elimination rate (r m) varied from 23 to 50 g m−3 h−1. Comparison between volumetric and biomass specific biodegradation activities indicated that toluene mass transfer was slower with PEG than with PUC as a consequence of a smaller biofilm surface and to the presence of larger zones of stagnant air.
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References
Beale EML (1960) Confidence regions in non-linear estimation. J R Stat Soc B22:41–88
Cox HHJ, Houtman JHM, Doddema HJ, Harder W (1993) Enrichment of fungi and degradation of styrene in biofilters. Biotechnol Lett 15:737–742
Devinny JS, Ramesh J (2005) A phenomenological review of biofilter models. Chem Eng J 113:187–196
Devinny JS, Deshusses MA, Webster TS (1999) Biofiltration for air pollution control. Lewis, Boca Raton
Dorado AD, Baquerizo G, Maestre JP, Gamisans X, Gabriel D, Lafuente J (2008) Modeling of a bacterial and fungal biofilter applied to toluene abatement: kinetic parameters estimation and model validation. Chem Eng J (in press). DOI https://doi.org/10.1016/j.cej.2007.09.004
Kennes C, Veiga MC (2001) Conventional biofilters. In: Kennes C, Veiga MC (eds) Bioreactors for waste gas treatment. Kluwer, Dordrecht, pp 47–98
Kennes C, Veiga MC (2004) Fungal biocatalysts in the biofiltration of VOC-polluted air. J Biotechnol 113:305–319
Kraakman NJR, Constanje R, Hamelers HVM (1997) Characterisation of air flow in biofilters. In: Prins WL, van Ham J (eds) Biological waste gas cleaning: proceedings of an international symposium. VDI Verlag, Maastricht, pp 215–218
Lee DH, Lau AK, Pinder KL (2001) Development and performance of an alternative biofilter system. J Air Waste Manage Assoc 51:78–85
Levenspiel O (1972) Chemical reaction engineering. Wiley, New York
Mendoza JA, Prado OJ, Veiga MC, Kennes C (2004) Hydrodynamic behaviour and comparison of technologies for the removal of excess biomass in gas-phase biofilters. Water Res 38:404–413
Moe WM, Irvine RL (2000) Polyurethane foam medium for biofiltration. II: operation and performance. J Environ Eng 126:826–832
Moe WM, Irvine RL (2001) Polyurethane foam based biofilter media for toluene removal. Water Sci Technol 43:35–42
Morgan-Sagastume JM, Noyola A (2006) Hydrogen sulfide removal by compost biofiltration: Effect of mixing the filter media on operational factors. Biores Technol 97:1546–1553
Morgan-Sagastume F, Sleep BE, Allen DG (2001) Effects of biomass growth on gas pressure drop in biofilters. J Environ Eng 127:388–396
Prenafeta-Boldú FX, Kuhn A, Luykx D, Anke H, van Groenestijn JW, de Bont JAM (2001) Isolation and characterisation of fungi growing on volatile aromatic hydrocarbons as their sole carbon and energy source. Mycol Res 105:477–484
Prenafeta-Boldú FX, Vervoort J, Grotenhuis JTC, van Groenestijn JW (2002) Substrate interactions during the biodegradation of benzene, toluene, ethylbenzene, and xylene (BTEX) hydrocarbons by the fungus Cladophialophora sp strain T1. Appl Environ Microbiol 68:2660–2665
Prenafeta-Boldú FX, Summerbell R, de Hoog GS (2006) Fungi growing on aromatic hydrocarbons: biotechnology's unexpected encounter with biohazard? FEMS Microbiol Rev 30:109–130
Sakuma T, Hattori T, Deshusses MA (2006) Comparison of different packing materials for the biofiltration of air toxics. J Air Waste Manage Assoc 56:1567–1575
van Groenestijn JW, Kraakman NJR (2005) Recent developments in biological waste gas purification in Europe. Chem Eng J 113:85–91
van Groenestijn JW, Liu JX (2002) Removal of a-pinene from gases using biofilters containing fungi. Atmos Environ 36:5501–5508
van Groenestijn JW, van Heiningen WNM, Kraakman NJR (2001) Biofilters based on the action of fungi. Water Sci Technol 44:227–232
Woertz JR, van Heiningen WNM, van Eekert MHA, Kraakman NJR, Kinney KA, van Groenestijn JW (2002) Dynamic bioreactor operation: effects of packing material and mite predation on toluene removal from off-gas. Appl Microbiol Biotechnol 58:690–694
Acknowledgements
This work has partly been funded by grants BIO4 CT 972295 from the European Commission and CTM2006-07976 from the Spanish Ministry of Science and Education. We acknowledge Dr. Guillermo Baquerizo Araya for his critical reading of the manuscript.
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Prenafeta-Boldú, F.X., Illa, J., van Groenestijn, J.W. et al. Influence of synthetic packing materials on the gas dispersion and biodegradation kinetics in fungal air biofilters. Appl Microbiol Biotechnol 79, 319–327 (2008). https://doi.org/10.1007/s00253-008-1433-2
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DOI: https://doi.org/10.1007/s00253-008-1433-2