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Production of d-lactic acid by Corynebacterium glutamicum under oxygen deprivation

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Abstract

In mineral salts medium under oxygen deprivation, Corynebacterium glutamicum exhibits high productivity of l-lactic acid accompanied with succinic and acetic acids. In taking advantage of this elevated productivity, C. glutamicum was genetically modified to produce d-lactic acid. The modification involved expression of fermentative d-lactate dehydrogenase (d-LDH)-encoding genes from Escherichia coli and Lactobacillus delbrueckii in l-lactate dehydrogenase (l-LDH)-encoding ldhA-null C. glutamicum mutants to yield strains C. glutamicum ΔldhA/pCRB201 and C. glutamicum ΔldhA/pCRB204, respectively. The productivity of C. glutamicum ΔldhA/pCRB204 was fivefold higher than that of C. glutamicum ΔldhA/pCRB201. By using C. glutamicum ΔldhA/pCRB204 cells packed to a high density in mineral salts medium, up to 1,336 mM (120 g l−1) of d-lactic acid of greater than 99.9% optical purity was produced within 30 h.

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Authors and Affiliations

  1. Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan

    Shohei Okino, Masako Suda, Keitaro Fujikura, Masayuki Inui & Hideaki Yukawa

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  1. Shohei Okino
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  2. Masako Suda
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  3. Keitaro Fujikura
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  4. Masayuki Inui
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  5. Hideaki Yukawa
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Correspondence to Shohei Okino.

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Okino, S., Suda, M., Fujikura, K. et al. Production of d-lactic acid by Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 78, 449–454 (2008). https://doi.org/10.1007/s00253-007-1336-7

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  • DOI: https://doi.org/10.1007/s00253-007-1336-7

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