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Influence of culture conditions on glutathione production by Saccharomyces cerevisiae

  • Biotechnological Products and Process Engineering
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Abstract

A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20–30°C), agitation rate (100–300 rpm), initial pH (5.0–7.0), inoculum concentration (5–15%), and glucose concentration (30–70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (25–2), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (22) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20°C; initial pH, 5; glucose, 54 g/l; and inoculum concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation.

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Acknowledgements

The authors would like to thank the financial support from The National Council for Scientific and Technological Development (CNPq) of Brazil.

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Authors and Affiliations

  1. Department of Food Engineering, College of Food Engineering, UNICAMP (State University of Campinas), Cx P. 6121, 13083-862,, Campinas, São Paulo, Brazil

    Lucielen Oliveira Santos, Tatiane Araujo Gonzales, Beatriz Torsani Úbeda & Ranulfo Monte Alegre

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  1. Lucielen Oliveira Santos
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  2. Tatiane Araujo Gonzales
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  3. Beatriz Torsani Úbeda
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  4. Ranulfo Monte Alegre
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Correspondence to Lucielen Oliveira Santos.

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Santos, L.O., Gonzales, T.A., Úbeda, B.T. et al. Influence of culture conditions on glutathione production by Saccharomyces cerevisiae . Appl Microbiol Biotechnol 77, 763–769 (2007). https://doi.org/10.1007/s00253-007-1211-6

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  • DOI: https://doi.org/10.1007/s00253-007-1211-6

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