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Acid phosphatase production by recombinant Arxula adeninivorans

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Abstract

Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett–Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these were optimized using response surface methodology of central composite design. The highest enzyme yields were attained in the medium with 3.9% sucrose and 1.6% peptone at pH 3.8. Because of optimization, 3.86- and 4.19-fold enhancement in enzyme production was achieved in shake flasks (17,054 U g−1 DYB) and laboratory fermenter (18,465 U g−1 DYB), respectively.

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Acknowledgments

The research work was supported by grants from DAAD (Grant no. KunzD04/34139) and DST (TS-INT/DAAD/P-108/2004), a DAAD scholarship (A/04/06850), and funds from the Chemical Industry (GK).

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Authors and Affiliations

  1. Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Neha Minocha, Parvinder Kaur & T. Satyanarayana

  2. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466, Gatersleben, Germany

    G. Kunze

Authors
  1. Neha Minocha
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  2. Parvinder Kaur
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  3. T. Satyanarayana
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  4. G. Kunze
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Correspondence to G. Kunze.

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Minocha, N., Kaur, P., Satyanarayana, T. et al. Acid phosphatase production by recombinant Arxula adeninivorans . Appl Microbiol Biotechnol 76, 387–393 (2007). https://doi.org/10.1007/s00253-007-1021-x

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  • DOI: https://doi.org/10.1007/s00253-007-1021-x

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