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A unique β-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303

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Abstract

The agaA gene encoding β-agarase-a (AgaA) was cloned from the chromosomal DNA of a marine bacterium, Vibrio sp. strain PO-303. The nucleotide sequence of the agaA gene consists of 2,958 bp and encodes a protein of 985 amino acids with a molecular mass of 106,062 Da. The deduced enzyme protein contains a typical N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2 (Big-2)-like domain of 52 amino acid residues, two carbohydrate-binding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. AgaA is the first agarase that was identified to possess a Big-2-like domain. The recombinant AgaA (rAgaA) expressed in Escherichia coli exhibited maximal activity around 40°C and pH 7.5, with a specific activity of 16.4 units mg−1, a K m of 1.10 mg ml−1, and a V max of 22.5 μmol min−1 mg−1 for agarose. The rAgaA hydrolyzed neoagarohexaose, but did not act on neoagarotetraose and neoagarobiose.

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Acknowledgment

This study was supported by a grant program of the Agriculture, Forestry and Fisheries Research Council of Japan (Research Project for Utilizing Advanced Technologies in Agriculture, Forestry, and Fisheries no. 1681, 2004–2006).

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  1. Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan

    Jinhua Dong, Yutaka Tamaru & Toshiyoshi Araki

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  1. Jinhua Dong
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  2. Yutaka Tamaru
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  3. Toshiyoshi Araki
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Correspondence to Toshiyoshi Araki.

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Dong, J., Tamaru, Y. & Araki, T. A unique β-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303. Appl Microbiol Biotechnol 74, 1248–1255 (2007). https://doi.org/10.1007/s00253-006-0781-z

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  • DOI: https://doi.org/10.1007/s00253-006-0781-z

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