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Fungal bioconversion of toxic polychlorinated biphenyls by white-rot fungus, Phlebia brevispora

  • Environmental Biotechnology
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Abstract

Toxic coplanar polychlorinated biphenyls (Co-PCBs) were used as substrates for a degradation experiment with white-rot fungus, Phlebia brevispora TMIC33929, which is capable of degrading polychlorinated dibenzo-p-dioxins. Eleven PCB congener mixtures (7 mono-ortho- and 4 non-ortho-PCBs) were added to the cultures of P. brevispora and monitored by high resolution gas chromatography and mass spectrometry (HRGC/HRMS). Five PCB congeners, 3,3′,4,4′-tetrachlorobiphenyl, 2,3,3′,4,4′-pentachlorobiphenyl, 2,3′,4,4′,5-pentachlorobiphenyl, 3,3′,4,4′,5-pentachlorobiphenyl, and 2,3′,4,4′,5,5′-hexachlorobiphenyl were degraded by P. brevispora. To investigate the fungal metabolism of PCB, each Co-PCB was treated separately by P. brevispora and the metabolites were analyzed by gas chromatography and mass spectrometry (GC/MS) and identified on the basis of the GC/MS comparison with the authentic compound. Meta-methoxylated metabolite was detected from the culture containing each compound. Additionally, para-dechlorinated and -methoxylated metabolite was also detected from the culture with 2,3,3′,4,4′-pentachlorobiphenyl, 2,3′,4,4′,5-pentachlorobiphenyl, and 2,3′,4,4′,5,5′-hexachlorobiphenyl, which are mono-ortho-PCBs. In this paper, we identified the congener specific degradation of coplanar PCBs by P. brevispora, and clearly proved for the first time by identifying the metabolites that the white-rot fungus, P. brevispora, transformed recalcitrant coplanar PCBs.

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Acknowledgements

We thank Shigeyuki Takagi and Ryusuke Higuchi (Azabu University, Kanagawa, Japan) for the technical assistance. This work was supported in part by a Grant-in-aid (hazardous chemicals) from the Ministry of Agriculture, Forestry, and Fisheries of Japan (HC-06-2444-1) and by a Grant-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan (16380121).

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Authors and Affiliations

  1. Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan

    Ichiro Kamei & Ryuichiro Kondo

  2. Graduate School of Environmental Health, Azabu University, 229-8501, Sagamihara, Kanagawa, Japan

    Shigenori Sonoki

  3. Daiichi College of Pharmaceutical Sciences, 22-1, Tamagawa-cho, 815-8511, Minamiku, Fukuoka, Japan

    Koichi Haraguchi

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  1. Ichiro Kamei
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  2. Shigenori Sonoki
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  3. Koichi Haraguchi
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  4. Ryuichiro Kondo
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Correspondence to Ryuichiro Kondo.

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Kamei, I., Sonoki, S., Haraguchi, K. et al. Fungal bioconversion of toxic polychlorinated biphenyls by white-rot fungus, Phlebia brevispora . Appl Microbiol Biotechnol 73, 932–940 (2006). https://doi.org/10.1007/s00253-006-0529-9

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  • DOI: https://doi.org/10.1007/s00253-006-0529-9

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