Skip to main content
SpringerLink
Log in

Amplification of 1-deoxy-d-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli

  • Applied Genetics and Molecular Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

For the enhancement of coenzyme Q10 (CoQ10) production, 1-deoxy-d-xylulose 5-phosphate (DXP) synthase of Pseudomonas aeruginosa was constitutively coexpressed in a recombinant Escherichia coli strain, which harbors the ddsA gene from Gluconobacter suboxydans encoding decaprenyl diphosphate synthase. It was found that the expression of the ddsA gene caused depletion of the isopentenyl diphosphate (IPP) pool in E. coli. Amplification of DXP synthase level by installing P. aeruginosa DXP synthase restored the diminished IPP pool and concomitantly resulted in approximately a twofold increase in relative content and productivity of CoQ10. Maximum CoQ10 concentration of 46.1 mg l−1 was achieved from glucose-limited fed-batch cultivation of the recombinant E. coli strain simultaneously harboring the ddsA and dxs genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Altincicek B, Duin EC, Reichenberg A, Hedderich R, Kollas AK, Hintz M, Wagner S, Wiesner J, Beck E, Jomaa H (2002) LytB protein catalyzes the terminal step of the 2-C-methyl-d-erythritol-4-phosphate pathway of isoprenoid biosynthesis. FEBS Lett 532:437–440

    Article  CAS  Google Scholar 

  • Choi JH, Ryu YW, Seo JH (2005) Biotechnological production and applications of coenzyme Q10. Appl Microbiol Biotechnol 68:9–15

    Article  CAS  Google Scholar 

  • Estévez JM, Cantero A, Reindl A, Reichler S, León P (2001) 1-Deoxy-d-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants. J Biol Chem 276:22901–22909

    Article  Google Scholar 

  • Harker M, Bramley PM (1999) Expression of prokaryotic 1-deoxy-d-xylulose-5-phosphatases in Escherichia coli increases carotenoids and ubiquinone biosynthesis. FEBS Lett 448:115–119

    Article  CAS  Google Scholar 

  • Kawamukai M (2002) Biosynthesis, bioproduction and novel roles of ubiquinone. J Biosci Bioeng 94:511–517

    Article  CAS  Google Scholar 

  • Kim SW, Keasling JD (2001) Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng 72:408–415

    Article  CAS  Google Scholar 

  • Kuzuyama T (2002) Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units. Biosci Biotechnol Biochem 66:1619–1627

    Article  CAS  Google Scholar 

  • Kuzuyama T, Takagi M, Takahashi S, Seto H (2000) Cloning and characterization of 1-deoxy-d-xylulose 5-phosphate synthase from Streptomyces sp. strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis. J Bacteriol 182:891–897

    Article  CAS  Google Scholar 

  • Lipshutz BH, Mollard P, Pfeiffer SS, Chrisman W (2002) A short, highly efficient synthesis of coenzyme Q10. J Am Chem Soc 124:14282–14283

    Article  CAS  Google Scholar 

  • Lois LM, Campos N, Rosa Putra S, Danielsen K, Rohmer M, Boronat A (1998) Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of d-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxal biosynthesis. Proc Natl Acad Sci U S A 95:2105–2110

    Article  CAS  Google Scholar 

  • Lois LM, Rodríguez-Concepción M, Gallego F, Campos N, Boronat A (2000) Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-d-xylulose 5-phosphate synthase. Plant J 22:503–513

    Article  CAS  Google Scholar 

  • Matthews PD, Wurtzel ET (2000) Metabolic engineering of carotenoids accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase. Appl Microbiol Biotechnol 53:396–400

    Article  CAS  Google Scholar 

  • Negishi E, Liou SY, Xu C, Huo S (2002) A novel, highly selective, and general methodology for the synthesis of 1,5-diene-containing oligoisoprenoids of all possible geometrical combinations exemplified by an iterative and convergent synthesis of coenzyme Q10. Org Lett 4:261–264

    Article  CAS  Google Scholar 

  • Nichols BP, Green JM (1992) Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase. J Bacteriol 174:5309–5316

    Article  Google Scholar 

  • Park YC, Kim SJ, Choi JH, Lee WH, Park KM, Kawamukai M, Ryu YW, Seo JH (2005) Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans. Appl Microbiol Biotechnol 67:192–196

    Article  CAS  Google Scholar 

  • Szkopiñska A (2000) Ubiquinone. Biosynthesis of quinone ring and its isoprenoid side chain. Intracellular localization. Acta Biochim Pol 47:469–480

    Article  Google Scholar 

  • Yoshida H, Katani Y, Ochiai K, Araki K (1998) Production of ubiquinone-10 using bacteria. J Gen Appl Microbiol 44:19–26

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by Korea Ministry of Commerce, Industry and Trade, and Ministry of Education through the BK21 program and in part by Korea Research Foundation Grant funded by Korean government (KRF-2004-005-F00055).

Author information

Authors and Affiliations

  1. Department of Agricultural Biotechnology and Research Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-921, South Korea

    Soo-Jung Kim, Jin-Ho Choi & Jin-Ho Seo

  2. BioNgene Co., Ltd., Seoul, 110-521, South Korea

    Sang-Yong Kim

  3. Department of Molecular Science and Technology, Ajou University, Suwon, 442-749, South Korea

    Yeon-Woo Ryu

  4. School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, 200-701, South Korea

    Myoung-Dong Kim

Authors
  1. Soo-Jung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Myoung-Dong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin-Ho Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sang-Yong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yeon-Woo Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jin-Ho Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Ho Seo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, SJ., Kim, MD., Choi, JH. et al. Amplification of 1-deoxy-d-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli . Appl Microbiol Biotechnol 72, 982–985 (2006). https://doi.org/10.1007/s00253-006-0359-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-006-0359-9

Keywords

Search

Navigation