Abstract
Human serum paraoxonase 1 (hPON1) belongs to a family of enzymes that catalyze the hydrolysis of a broad range of esters and lactones. Although the very first identification of hPON1 might have been as a calcium-dependent paraoxonase/arylesterase, PON1 is in fact a lactonase associated with high-density lipoprotein and strongly stimulated by apoA-I. PON1 hydrolyzes various organophosphates, including insecticides and nerve gases. PON1 also plays a key role in prevention of atherosclerosis. Mediation of cholesterol efflux from macrophage is a key in vivo function of PON1. In present study, the hPON1 Q gene was cloned into baculovirus transfer vector pVL1392 and expressed in silkworm expression system. The rhPON1 Q presented two bands with every near molecular weight of about 40 and 43 kDa according to sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis. The expression level was up to 1,256 mg/L in haemolymph, about 50 times as high as that from BmN cells (24.8 mg/L). After purified by two chromatography steps (DEAE-Sepharose and HiTrap Chelating HP), the purity of rhPON1 Q was up to 90%, and the enzymatic properties are similar to serum hPON1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS (2004) Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization. Proc Natl Acad Sci U S A 101(2):482–487
Broomfield CA, Ford KW (1991) Hydrolysis of nerve gases by plasma enzymes. In: Proceedings of the 3rd international meeting on cholinesterases, La Grande-Motte, France, p 161
Brushia RJ, Forte TM, Oda MN, La Du BN, Bielicki JK (2001) Baculovirus-mediated expression and purification of human serum paraoxonase 1A. J Lipid Res 42:951–958
Draganov DI, La Du BN (2004) Pharmacogenetics of paraoxonases: a brief review. Naunyn Schmiedebergs Arch Pharmacol 369:78–88
Draganov DI, Teiber JF, Speelman A, Osawa Y, Sunahara R, La Du BN (2005) Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. J Lipid Res 46:1239–1247
Durrington PN, Mackness B, Mackness MI (2001) Paraoxonases and atherosclerosis. Arterioscler Thromb Vasc Biol 21:473–480
Gaidukov L, Tawfik DS (2005) High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I. Biochemistry 44:11843–11854
Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RBG, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS (2004) Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzyme. Nat Struct Mol Biol 11:412–419
Harley RD, Billecke S, La Du BN (1999) Association of low PON1 type Q (type A) arylesterase activity with neurologic symptom complexes in Gulf War veterans. Toxicol Appl Pharmacol 157:233–239
Imai Y, Morita H, Kurihara H, Sugiyama T, Kato N, Ebihara A, Hamada C, Kurihara Y, Shindo T, Oh-hashi Y, Yazaki Y (2000) Evidence for association between paraoxonase gene polymorphisms and atherosclerotic diseases. Atherosclerosis 149:435–442
Khersonsky O, Tawfik DS (2005) Structure–reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase. Biochemistry 44:6371–6382
La Du BN, Kalow W (1992) Human serum paraoxonase/arylesterase. Genetic factors influencing the metabolism of foreign compounds. International encyclopedia of pharmacology and therapeutics. Pergamon, New York, pp 51–91
La Du BN, Adkins S, Chung-Liang AK, Lipsig D (1993) Studies on human serum paraoxonase/arylesterase. Chem Biol Interact 87:25–34
Peng R, Yu Z, Zang Y, Qin J (1999) High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm. Biotechnol Lett 21:979–985
Qiu P, Ding Y, Qin J, Han K, Zhu D (1994) Expression of biologically active monomeric form of human M-CSF in baculovirus infected silkworm, Bombyx mori. Biol Chem Hoppe Seyler 375:413–418
Rosenblat M, Vayab J, Shihc D, Avirama M (2005) Paraoxonase 1 (PON1) enhances HDL-mediated macrophage cholesterol efflux via the ABCA1 transporter in association with increased HDL binding to the cells: a possible role for lysophosphatidylcholine. Atherosclerosis 179:69–77
Shi X, Qin J, Zhu J, Zhu D (1996) Expression of biologically active human granulocyte-macrophage colony-stimulating factor in the silkworm (Bombyx mori). Biotechnol Appl Biochem 24:245–249
Shih DM, Gu L, Xia YR, Navab M, Li WF, Hama S, Castellani LW, Furlong CE, Costa LG, Fogelman AM, Lusis AJ (1998) Mice lacking serum paraoxonase are susceptible to organophosphate toxicity and atherosclerosis. Nature 394:284–287
Sorenson RC, Bisagaier CL, Aviram M, Hsu C, La Du BN (1999) Human serum paraoxonase/arylesterase’s retained hydrophobic N-terminal leader sequence associates with HDLs by binding phospholipids: apolipoprotein A-I stabilizes activity. Arterioscler Thromb Vasc Biol 19:2214–2225
Vitarius JA, Sultatos LG (1995) The role of calcium in the hydrolysis of the organophosphate paraoxon by human serum A-esterase. Life Sci 56:125–134
Wang L, Qin J, Shen B, Zhu J, Zang Y (1995) Expression of human stem cell factor in the baculoviruses expression vector system. Biochem Mol Biol Int 37:729–736
Acknowledgement
The project was supported by State Key Laboratory of Pharmaceutical Biotechnology.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhu, J., Ze, Y., Zhang, C. et al. High-level expression of recombinant human paraoxonase 1 Q in silkworm larvae (Bombyx mori). Appl Microbiol Biotechnol 72, 103–108 (2006). https://doi.org/10.1007/s00253-005-0246-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00253-005-0246-9