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Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

  • Applied Microbial and Cell Physiology
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Abstract

Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism.

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Acknowledgements

Ms Jenny Ågren (Lund University) and Mr Henk Blignault (Stellenbosch University) are gratefully acknowledged for technical assistance during screening of the medium components.

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Authors and Affiliations

  1. Department of Applied Microbiology, Lund University, Box 124, 221 00, Lund, Sweden

    Johann F. Görgens & Bärbel Hahn-Hägerdal

  2. Department of Process Engineering, University of Stellenbosch, South Africa

    Johann F. Görgens & Johannes H. Knoetze

  3. Department of Microbiology, University of Stellenbosch, South Africa

    Willem H. van Zyl

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  1. Johann F. Görgens
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  2. Willem H. van Zyl
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  3. Johannes H. Knoetze
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  4. Bärbel Hahn-Hägerdal
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Correspondence to Bärbel Hahn-Hägerdal.

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Görgens, J.F., van Zyl, W.H., Knoetze, J.H. et al. Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium. Appl Microbiol Biotechnol 67, 684–691 (2005). https://doi.org/10.1007/s00253-004-1803-3

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