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Effects of medium composition on MFα1 promoter-directed secretion of a small protease inhibitor in Saccharomyces cerevisiae batch fermentation

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Summary

Casaminoacid supplementation of yeast nitrogen base (YNB) medium is widely used in the culture of recombinant yeasts as it enables respiratory growth on YNB and selection for URA3- or TRP1-bearing plasmids. Despite these advantages, this supplementation to YNB medium resulted in marked decreases in cellular productivity for the secretion of a protease inhibitor product in batch fermentation.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University College London, WC1E 6BT, London, UK

    Yaping Chen, Niall Kirk & Peter W. Piper

  2. Department of Biochemical Engineering, University College London, WC1E 6BT, London, UK

    Niall Kirk

Authors
  1. Yaping Chen
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  2. Niall Kirk
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  3. Peter W. Piper
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Chen, Y., Kirk, N. & Piper, P.W. Effects of medium composition on MFα1 promoter-directed secretion of a small protease inhibitor in Saccharomyces cerevisiae batch fermentation. Biotechnol Lett 15, 223–228 (1993). https://doi.org/10.1007/BF00128309

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