Abstract
This chapter describes some of the available methods to assess EPS production in bifidobacteria, being largely based on those developed for the same purpose for members of the lactic acid bacteria group. The first step is detection of putative EPS-producing bifidobacteria based on a mucoid and/or ropy phenotype. Next, a basic procedure is described for the isolation of the glycan polymer based on the release from bifidobacterial cells grown and collected from the surface of agar-MRSc (“crude EPS”), followed by a purification procedure intended to remove other bacterial macromolecules (DNA and proteinaceous material) to generate “purified EPS.” Finally, several methods used for quantification and physical–chemical characterization of isolated/purified polysaccharide are outlined.
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Acknowledgments
Research on EPS from Bifidobacterium and LAB in our group has been in supported by long-term funding from the National Plans of Research, Development and Innovation (“I+D+i”) of the Spanish Ministry of Science (reference of the current one: RTI2018-096339-B-I00). The “I+D+i” Regional from Plan the Government of the “Principado de Asturias” also funds the research activities (period 2018–2020) of the MicroHealth group (reference IDI/2018/000236). Both projects are partially cofunded by the FEDER (European Regional Development Fund) program from the European Union. P. Ruas-Madiedo is grateful to the Ph.D. students that have worked or are still working on this topic during their doctoral Thesis, as well as to the excellent technical assistance of Isabel Cuesta for the chromatographic analyses.
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Ruas-Madiedo, P. (2021). Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides. In: van Sinderen, D., Ventura, M. (eds) Bifidobacteria. Methods in Molecular Biology, vol 2278. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1274-3_9
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