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Degradation of polycyclic aromatic hydrocarbons by a newly isolated dibenzofuran-utilizing Janibacter sp. strain YY-1

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Abstract

The dibenzofuran (DF)-utilizing bacterium strain YY-1 was newly isolated from soil. The isolate was identified as Janibacter sp. with respect to its 16S rDNA sequence and fatty acid profiles, as well as various physiological characteristics. In addition to DF, strain YY-1 could grow on fluorene and dibenzothiophene as sole sources of carbon and energy. It was also able to cometabolize a variety of polycyclic aromatic hydrocarbons including dibenzo-p-dioxin, phenanthrene, and anthracene. The major metabolites formed from DF, biphenyl, dibenzothiophene, and naphthalene were identified by using gas chromatography-mass spectrometry as 2,3,2′-trihydroxybiphenyl, biphenyl-dihydrodiol, dibenzothiophene 5-oxide, and coumarin, respectively. These results indicate that strain YY-1 can catalyze angular dioxygenation, lateral dioxygenation, and sulfoxidation.

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Acknowledgment

We thank Akira Yokota (Institute of Molecular and Cellular Biosciences, University of Tokyo) for valuable advice and for providing us with the MIDI system used in the identification of strain YY-1.

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Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113–8657, Tokyo , Japan

    A. Yamazoe & H. Oyaizu

  2. Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113–8657, Tokyo , Japan

    A. Yamazoe & H. Oyaizu

  3. Research Center for Water Environment Technology, The University of Tokyo, Bunkyo-ku, 113–8657, Tokyo , Japan

    O. Yagi

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  1. A. Yamazoe
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  2. O. Yagi
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Correspondence to A. Yamazoe.

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Yamazoe, A., Yagi, O. & Oyaizu, H. Degradation of polycyclic aromatic hydrocarbons by a newly isolated dibenzofuran-utilizing Janibacter sp. strain YY-1. Appl Microbiol Biotechnol 65, 211–218 (2004). https://doi.org/10.1007/s00253-003-1541-y

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  • DOI: https://doi.org/10.1007/s00253-003-1541-y

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