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The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe

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Abstract

The response of Schizosaccharomyces pombe towards the oxyanions selenate [Se(VI)] and dichromate [Cr(VI)] was investigated in order to establish the involvement of the yeast ATP sulfurylase in their reduction. An ATP sulfurylase-defective/selenate-resistant mutant of S. pombe (B-579 Se R -2) and an ATP sulfurylase-active/selenate-sensitive strain of S. pombe (B-579 Se S) were included in this study. The inhibitory effect of Se(VI) and Cr(VI) oxyanions on growth and bioaccumulation was measured. The sensitive strain showed natural sensitivity to selenate while the resistant mutant tolerated a 100-fold higher concentration of selenate. These results indicate that selenate toxicity to microorganisms is connected with the reduction of selenate to selenite. Both strains showed similar sensitivity to Cr(VI) and in this study there was no evidence that ATP sulfurylase participates in the reduction process of Cr(VI).

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Acknowledgements

This study was supported by The Ministry of Education, Science and Sport, Republic of Slovenia (project No. J4-7454-490) and The Ministry of Agriculture, Forests and Food, Republic of Slovenia (project No. V4-0402). The authors are grateful to Dr. Radmila Milačič for carrying out the FAAS analysis and Dr. Vekoslava Stibilj, who enabled our HG-AFS analysis.

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Authors and Affiliations

  1. Biotechnical Faculty, Department of Food Science and Technology, Chair of Biotechnology, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia

    P. Raspor, Š. Fujs & M. Batič

  2. Faculty of Food Science, Department of Microbiology and Biotechnology, Szent Istvan University, Somlói út. 14–16, 1118 , Budapest, Hungary

    L. Banszky & A. Maraz

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  1. P. Raspor
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  2. Š. Fujs
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  3. L. Banszky
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  4. A. Maraz
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  5. M. Batič
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Correspondence to P. Raspor.

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Raspor, P., Fujs, Š., Banszky, L. et al. The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe . Appl Microbiol Biotechnol 63, 89–95 (2003). https://doi.org/10.1007/s00253-003-1336-1

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  • DOI: https://doi.org/10.1007/s00253-003-1336-1

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