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Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281

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Abstract

The influence of residual ethanol on metabolism of food grade Gluconacetobacter xylinus I 2281 was investigated during controlled cultivations on 35 g/l glucose and 5 g/l ethanol. Bacterial growth was strongly reduced in the presence of ethanol, which is unusual for acetic acid bacteria. Biomass accumulated only after complete oxidation of ethanol to acetate and carbon dioxide. In contrast, bacterial growth initiated without delay on 35 g/l glucose and 5 g/l acetate. It was found that acetyl CoA was activated by the acetyl coenzyme A synthetase (Acs) pathway in parallel with the phosphotransacetylase (Pta)-acetate kinase (Ack) pathway. The presence of ethanol in the culture medium strongly reduced Pta activity while Acs and Ack remained active. A carbon balance calculation showed that the overall catabolism could be divided into two independent parts: upper glycolysis linked to glucose catabolism and lower glycolysis liked to ethanol catabolism. This calculation showed that the carbon flux through the tricarboxylic cycle is lower on ethanol than on acetate. This corroborated the diminution of carbon flux through the Pta-Ack pathway due to the inhibition of Pta activity on ethanol.

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Acknowledgements

Financial support from the Swiss Commission for Technology and Innovation CTI 4491.2 is gratefully acknowledged. Experiments presented in this study comply with the current laws of Switzerland.

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Authors and Affiliations

  1. Laboratory of Chemical and Biological Engineering, Institute of Chemical Engineering, Swiss Federal Institute of Technology Lausanne, 1015, Lausanne, Switzerland

    H. Kornmann, I. Marison & U. von Stockar

  2. Nestlé Research Center, 1000 , Lausanne 26, Switzerland

    P. Duboc & P. Niederberger

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  1. H. Kornmann
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  2. P. Duboc
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  3. P. Niederberger
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  4. I. Marison
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  5. U. von Stockar
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Correspondence to U. von Stockar.

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Kornmann, H., Duboc, P., Niederberger, P. et al. Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281. Appl Microbiol Biotechnol 62, 168–173 (2003). https://doi.org/10.1007/s00253-003-1299-2

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  • DOI: https://doi.org/10.1007/s00253-003-1299-2

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