Abstract.
The microorganisms Trichosporon cutaneum and Bacillus licheniformis were used to develop a microbial biochemical oxygen demand (BOD) sensor. It was found that T. cutaneum gave a greater response to glucose, whereas B. licheniformis gave a better response to glutamic acid. Hence, co-immobilized T. cutaneum and B. licheniformis were used to construct a glucose and glutamic acid sensor with improved sensitivity and dynamic range. A membrane loading of T. cutaneum at 1.1×108 cells ml–1 cm–2 and B. licheniformis at 2.2×108 cells ml–1 cm–2 gave the optimum result: a linear range up to 40 mg BOD l–1 with a sensitivity of 5.84 nA mg–1 BOD l. The optimized BOD sensor showed operation stability for 58 intermittent batch measurements, with a standard deviation of 0.0362 and a variance of 0.131 nA. The response time of the co-immobilized microbial BOD sensor was within 5–10 min by steady-state measurement and the detection limit was 0.5 mg BOD l–1. The BOD sensor was insensitive to pH in the range of pH 6.8–7.2.
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Suriyawattanakul, .L., Surareungchai, .W., Sritongkam, .P. et al. The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor. Appl Microbiol Biotechnol 59, 40–44 (2002). https://doi.org/10.1007/s00253-002-0980-1
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DOI: https://doi.org/10.1007/s00253-002-0980-1