Abstract
New chicken Rfp-Y haplotypes were determined by the use of restriction fragment length polymorphism (RFLP) and mixed lymphocyte culture (MLC) in four different chicken haplotypes, B 15, B 19, B 21, B 201. The RFLP polymorphism was mapped to the Rfp-Y system by the use of a subclone (18.1) which maps near a polymorphic lectin gene located in the Rfp-Y system and DNA from families with known segregation of the implicated RFLP polymorphism. For the first time it is shown that major histocompatibility complex class II genes in the Rfp-Y system have functional implications. Sequence information of the B1 domain of the proposed Rfp-Y haplotypes was obtained which supported the functional data.
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Received: 30 August 1996 / Revised: 31 December 1996
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Juul-Madsen, H., Zoorob, R., Auffray, C. et al. New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses. Immunogenetics 45, 345–352 (1997). https://doi.org/10.1007/s002510050215
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DOI: https://doi.org/10.1007/s002510050215