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Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells

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Abstract

To understand the biology of γδ T cells in ruminants, it is necessary to have a comprehensive picture of γδ T-cell receptor gene diversity and expression. In this study, three new subgroups of bovine T-cell receptor δ (TRD) variable genes were identified by RT-PCR and sequencing and homology with TRDV genes from other mammals determined. Previously unidentified TRDV subgroup genes described in this study include the bovine homologues of ovine TRDV2, TRDV3, and TRDV4 which were named accordingly. TRDV2 subgroup has two genes (TRDV2-1 and TRDV2-2) while we found the previously identified TRDV1 has at least eight genes corresponding to separate genomic sequences. Nucleotide and amino acid sequences for particular gene subgroups between cattle and sheep were more than 87% identical but identities among TRDV subgroups within a species were much less, with bovine TRDV4 having <45% identity to the other three bovine TRDV gene subgroups. Analysis of circulating bovine γδ T cells revealed that genes from all four TRDV subgroups were expressed in combination with TRDJ1, TRDJ3, and TRDC, although TRDV4 was the least represented, and all displayed a variety of CDR3 junctional lengths. Finally, some genes within the TRDV1, TRDV2, and TRDV3 subgroups recombined with TRAV incorporating TRAJs, suggesting dual use.

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Acknowledgements

This work was supported by the National Research Initiative of the USDA CSREES, grant #2002-02038 and 2004-01157, and the Massachusetts Agricultural Experiment Station Project No. MAS00851. Dr. Marie-Paule Lefranc is gratefully acknowledged for her assistance in gaining approval for the names of new bovine genes and Dr. Janice Telfer for the advice in quantitation of PCR products.

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Author notes

  1. Seth L. Blumerman

    Present address: Trudeau Institute, Saranac Lake, NY, USA

Authors and Affiliations

  1. Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, Amherst, MA, 01003, USA

    Carolyn T. A. Herzig, Seth L. Blumerman & Cynthia L. Baldwin

  2. Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA, 01003, USA

    Cynthia L. Baldwin

Authors
  1. Carolyn T. A. Herzig
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  2. Seth L. Blumerman
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  3. Cynthia L. Baldwin
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Corresponding author

Correspondence to Cynthia L. Baldwin.

Additional information

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank database under the accession numbers DQ275147, DQ275148, DQ275149, and DQ280318.

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Herzig, C.T.A., Blumerman, S.L. & Baldwin, C.L. Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells. Immunogenetics 58, 746–757 (2006). https://doi.org/10.1007/s00251-006-0136-z

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