Abstract
IgM and IgD genes of the Japanese flounder were cloned and characterized from a genomic bacterial artificial chromosome (BAC) library. The μ gene contained four constant region exons (Cμ1–Cμ4), and two transmembrane exons (μTM1 and μTM2), which conforms to the organizational pattern of all other vertebrate μ-chain genes examined so far. In the same BAC clone, the δ gene, which is homologous to the IgD gene in mammals and teleost fish, was found immediately (0.9 kb) downstream of the IgM gene. This gene encoded seven exons (Cδ1–Cδ7) and two δTM exons (δTM1 and δTM2) and had no duplication of Cδ1-Cδ2, as found in Atlantic cod, or Cδ2-Cδ3-Cδ4, as found in Atlantic salmon and channel catfish. Phylogenetic and sequence analyses strongly suggest that teleost δ is more closely related to non-IgM isotypes than IgM isotypes. The heavy chain (IgH) locus of Japanese flounder, which encodes μm, μs and δm, was found to be fully functional.
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This study was supported in part by Grants-in-Aid for Scientific Research (S) from the Ministry of Education, Science, Sports and Culture of Japan.
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Srisapoome, P., Ohira, T., Hirono, I. et al. Genes of the constant regions of functional immunoglobulin heavy chain of Japanese flounder, Paralichthys olivaceus. Immunogenetics 56, 292–300 (2004). https://doi.org/10.1007/s00251-004-0689-7
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DOI: https://doi.org/10.1007/s00251-004-0689-7