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Improved Nutrient Use Efficiency Increases Plant Growth of Rice with the Use of IAA-Overproducing Strains of Endophytic Burkholderia cepacia Strain RRE25

  • Plant Microbe Interactions
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Abstract

Effect of indole acetic acid (IAA)-overproducing mutants of Burkholderia cepacia (RRE25), a member of β-subclass of Proteobacteria and naturally occurring rice endophyte, was observed on the growth of rice (Oryza sativa L.) plants grown under greenhouse conditions. Nine mutants were characterized for altered biosynthesis of IAA after nitrous acid mutagenesis. These mutants were grouped into two classes: class I mutants have reduced production of IAA as compared to the wild type, while class II mutants showed overproduction of IAA. Mutants of both classes and RRE25, the parent (wild type), were inoculated on rice seedlings of two cultivars (Sarjoo-52 and NDR-97). Uptake of nitrogen, phosphorous, and potassium was estimated in these plants. Significant increase in the amount of uptake of all three elements was observed when inoculated with the IAA-overproducing mutants over control as well as in the plants inoculated with the wild type (RRE25). Effect of inoculation of IAA-overproducing mutants was more pronounced on the uptake of phosphorous in cultivar NDR-97 than Sarjoo-52, while it was opposite with respect to potassium uptake. Any significant difference was not observed in nitrogen uptake among the two cultivars. It shows that the host also plays an important role in the beneficial endophytic association. It was concluded from these results that one of the possible mechanisms of growth promotion of rice plants inoculated with bacterial endophytes is their effects on an increase in the capability of nutritional uptake possible through the effect of IAA production which results in proliferation of root system that could mine more nutrients from the soil.

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Acknowledgments

Authors are greatly thankful to the Department of Science and Technology, Government of India, for the financial assistance to carry out this part of the research work.

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Authors and Affiliations

  1. PGPR Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Ramesh K Singh & Namrata Malik

  2. Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Namrata Malik & Surendra Singh

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  1. Ramesh K Singh
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  2. Namrata Malik
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Correspondence to Ramesh K Singh.

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Singh, R.K., Malik, N. & Singh, S. Improved Nutrient Use Efficiency Increases Plant Growth of Rice with the Use of IAA-Overproducing Strains of Endophytic Burkholderia cepacia Strain RRE25. Microb Ecol 66, 375–384 (2013). https://doi.org/10.1007/s00248-013-0231-2

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