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Species Diversity, Distribution, and Genetic Structure of Endophytic and Epiphytic Trichoderma Associated with Banana Roots

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Abstract

Selective isolation, molecular identification and AFLP were used to investigate the distribution of the various species of endophytic and epiphytic Trichoderma associated with banana roots and to compare and contrast their genetic structure. Three specific groups of Trichoderma were observed in the roots of banana. Group one, which made up the largest population, comprised T. asperellum, T. virens, and Hypocrea lixii, which were isolated from both inside and on the surface of the banana roots, while group two, made up of T. atroviride and T. koningiopsis, existed on the surface only. Group three, comprising only T. brevicompactum was isolated from the inside of the roots. The AFLP analysis revealed Nei’s diversity indices of 0.15 and 0.26 for epiphytic T. asperellum and T. virens, respectively. The index values of 0.11 and 0.11 were obtained for endophytic T. asperellum and T. virens, respectively. The genetic diversity within endophytic T. asperellum and T. virens was lower than that within the epiphytes. This suggests that endophytic Trichoderma has a higher genetic conservation and is compatible with the relatively stable microenvironments inside roots.

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Acknowledgments

We are grateful for the support of National Natural Sciences Foundation of China (30500632), National High Technology Research and Development Program of China (2006AA10A02), and project (F) 2007DFA30970 of the Ministry of Science and Technology.

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Science, Xiamen University, Xiamen, 361005, People’s Republic of China

    Xiaomin Xia, Timothy K. Lie, Xiaoming Qian, Zhonghui Zheng, Yaojian Huang & Yuemao Shen

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  1. Xiaomin Xia
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  2. Timothy K. Lie
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  3. Xiaoming Qian
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  4. Zhonghui Zheng
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  5. Yaojian Huang
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Corresponding author

Correspondence to Yaojian Huang.

Electronic Supplementary Material

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Table S1

The identification of Trichoderma isolated from banana roots and their accession numbers in GenBank (PDF 48 kb)

Table S2

Genetic identity within endophytic T. virens and epiphytic T. virens (PDF 74 kb)

Table S3

Genetic identity within endophytic T. asperellum and epiphytic T. asperellum (PDF 17 kb)

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Xia, X., Lie, T.K., Qian, X. et al. Species Diversity, Distribution, and Genetic Structure of Endophytic and Epiphytic Trichoderma Associated with Banana Roots. Microb Ecol 61, 619–625 (2011). https://doi.org/10.1007/s00248-010-9770-y

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