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Reptiles as a Source of Salmonella O48—Clinically Important Bacteria for Children: The Relationship Between Resistance to Normal Cord Serum and Outer Membrane Protein Patterns

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Abstract

Bacteria of the Salmonella O48 somatic antigen group are clinically important strains causing intestinal dysfunction and diarrhoea, especially in children. The susceptibility of Salmonella O48 strains containing sialic acid (N-acetylneuraminic acid (NeuAc)) in lipopolysaccharide (LPS) to the bactericidal action of normal cord serum (NCS) was determined. The authors' previous results published in Microbial Ecology in 2010 indicated that neither the presence of NeuAc in LPS nor the length of the O-specific part of LPS containing NeuAc plays a decisive role in determining bacterial resistance to the bactericidal activity of normal human serum (NHS), and that the presence of NeuAc in the LPS structure is not sufficient to block the activation of the alternative pathway of complement in NHS. The current results showed that the tested strains showed various sensitivities also to the bactericidal action of NCS. The authors postulate that the presence of certain outer membrane proteins (OMPs) are characteristic of the resistant and sensitive phenotypes of Salmonella O48 strains. To establish a possible relationship between resistance to NCS and OMPs band patterns, ten Salmonella O48 strains were studied as follows: susceptibility to the bactericidal effect of NCS, the mechanisms of NCS activation and OMP band patterns obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

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Acknowledgments

The authors thank Prof. Dr. hab. A. Gamian (Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland) for the Salmonella strains from the Polish Collection of Microorganisms. We would like to thank Mgr. Aleksandra Skwara for the contribution to figure preparation.

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Authors and Affiliations

  1. Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, 51-148, Wrocław, Poland

    Gabriela Bugla-Płoskońska & Katarzyna Guz-Regner

  2. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wrocław, Poland

    Agnieszka Korzeniowska-Kowal

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  1. Gabriela Bugla-Płoskońska
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  2. Agnieszka Korzeniowska-Kowal
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Correspondence to Gabriela Bugla-Płoskońska.

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Bugla-Płoskońska, G., Korzeniowska-Kowal, A. & Guz-Regner, K. Reptiles as a Source of Salmonella O48—Clinically Important Bacteria for Children: The Relationship Between Resistance to Normal Cord Serum and Outer Membrane Protein Patterns. Microb Ecol 61, 41–51 (2011). https://doi.org/10.1007/s00248-010-9677-7

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