Abstract
Ozone is considered as the main factor in air pollution related to a decline of forest in North America and Europe. In the present study, the effect of changed litter quality, due to ozone stress to trees, on the microbial communities colonizing the subsequent litter was investigated. Litter bag technique using beech and spruce litter from ozone-stressed and control trees, was combined with 16S and 18S rRNA-based fingerprinting methods and cloning to characterize phylogenetic diversity. Litter bags were incubated for 2 and 8 weeks in a beech–spruce mixed forest. Differences between the structure of microbial communities colonizing control and ozone-exposed litter were evident by fingerprints of 16S and 18S rRNA RT-PCR products. RT-PCR products, from litter degraded for 8 weeks, were cloned to identify the bacterial and fungal groups. Clones similar to members of Actinobacteria dominated the bacterial libraries, whereas effects of changed litter quality were mainly observed for the Proteobacteria. Fungal libraries were dominated by clones similar to Ascomycota members. Reduced proportion of clones similar to Basidiomycota and Zygomycota in library from ozone-stressed spruce trees and Chytridiomycota from ozone-stressed beech trees was observed when compared to their control counterparts. As hypothesized, changed litter quality due to elevated O3 did influence the structure of litter-colonizing microbial communities. However, these differences were not as pronounced as those between the two plant species.
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Abrams, ES, Stanton, VP (1992) Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acids. Methods Enzymol 212: 71–105
Akasaka, H, Izawa, T, Ueki, K, Ueki, A (2003) Phylogeny of numerically abundant culturable anaerobic bacteria associated with degradation of rice plant residue in Japanese paddy field soil. FEMS Microbiol Ecol 43: 149–161
Andersen, CP (2003) Source–sink balance and carbon allocation below ground in plants exposed to ozone. New Phytol 157: 213–228
Aneja, MK, Sharma, S, Munch, JC, Schloter, M (2004) RNA fingerprinting—a new method to screen for differences in plant litter degrading microbial communities. J Microbiol Methods 59: 223–231
Aneja, MK, Sharma, S, Fleishmann, F, Stich, S, Heller, W, Bahnweg, G, Munch, JC, Schloter, M (2006) Microbial degradation of beech and spruce litter-influence of soil type and litter quality on the composition of microbial populations involved in the turnover process. Microb Ecol 52: 127–135
Baker, TR, Allen, HL, Schoenberger, MM, Kress, LW (1994) Nutritional response of loblolly pine exposed to ozone and simulated acid rain. Can J For Res 24: 453–461
Bruce, RJ, West, CA (1989) Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Plant Physiol 91: 889–897
Buchan, A, Newell, SY, Butler, M, Biers, EJ, Hollibaugh, JT, Moran, MA (2003) Dynamics of bacterial and fungal communities on decaying salt marsh grass. Appl Environ Microbiol 69: 6676–6687
Buchan, A, Newell, SY, Moreta, JI, Moran, MA (2002) Molecular characterization of bacterial and fungal decomposer communities in a southeastern U.S. saltmarsh. Microb Ecol 43: 329–340
Camel, V, Bermond, A (1998) The use of ozone and associated oxidation processes in drinking water treatment. Water Res 11: 3208–3222
Chappelka, AH, Chevone, BI (1992) Tree responses to ozone. In: Lefohn, AS (Ed.) Surface Level Ozone Exposures and Their Effects on Vegetation, Lewis Publishers, Chelsea, pp 271–324
Cox, P, Fischer, PJ, Anderson, JM (1997) Experiments in fungal survival of two common pine litter colonisers. Mycologia 11: 55–58
Edwards, GS, Kelly, JM, Mays, PA (1992) Ozone, acidic precipitation, and soil Mg impacts on soil and loblolly pine seedling nutrient status after three growing seasons. Water Air Soil Pollut 63: 281–294
Frankland, JC (1992) Mechanisms in fungal succession. In: Carroll, GC, Wicklow, DT (Eds.) The Fungal Community: Its Organisation and Role in the Ecosystem, Marcel Dekker, New York, pp 383–402
Heuer, H, Smalla, K (1997) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) for studying soil microbial communities. In: van Elsas, JD, Wellington, EMH, Trevors, JT (Eds.) Modern Soil Microbiology, Marcel Dekker, New York, pp 353–373
Heukeshoven, J, Dernick, R (1986) Neue Ergebnisse zum Mechanismus der Silberfaerbung. In: Radola, BJ (Ed.) Electrophorese Forum ’86, Technische Universitaet Muenchen, Munich, pp 22–27
Houghton, JT, Meira Filho, LG, Callander, BA, Harris, N, Kattenberg, A, Maskell, K (Eds.) (1996) Climate Change 1995: The Science of Climate Change, Cambridge University, Cambridge, UK, 572 pp
Lehtola, M, Miettinen, I, Vartiainen, T, Myllykangas, T, Martikainen, P (2001) Microbially available organic carbon, phosphorus and microbial growth in ozonated drinking water. Water Res 35: 1635–1640
Loranger, G, Pregitzer, K, King, JS (2004) Elevated CO2 and O3 concentrations differentially affect selected groups of the fauna in temperate forest soils. Soil Biol Biochem 36: 1521–1524
Maguire, DA (1994) Branch mortality and potential litter fall from Douglas-fir trees in stands of varying density. For Ecol Manag 70: 41–53
Manning, W, Tiedemann, A (1995) Climate change: potential effects of increased atmospheric carbon dioxide, ozone and ultraviolet-B radiation on plant diseases. Environ Pollut 33: 219–245
Miller, HG (1984) Dynamics of nutrient cycling in plantation ecosystems. In: Boven, GD, Nambiar, EKS (Eds.) Nutrition of Plantation Forest, Academic Press, London, pp 53–78
Newell, SY (2001) Multiyear patterns of fungal biomass dynamics and productivity within naturally decaying smooth cordgrass shoots. Limnol Oceanogr 46: 573–583
Newell, SY, Porter, D (2000) Microbial secondary production from saltmarsh grass shoots and its known potential fates. In: Sienstein, MP, Kreeger, DA (Eds.) Concepts and Controversies in Tidal Marsh Ecology, Kluwer Academic, Dordrecht, pp 159–185
Ohlenbusch, G, Hesse, S, Frimmel, FH (1998) Effects of ozone treatment on the soil organic matter on contaminated sites. Chemosphere 37: 1557–1569
Reich, PB, Schoettle, AW, Stroo, HF, Amundson, RG (1988) Effects of ozone and acid rain on white pine (Pinus strobus) seedlings grown in five soils III. Nutrient relations. Can J Bot 66: 1517–1531
Runeckles, VC, Chevone, BI (1992) Crop responses to ozone. In: Lefohn, AS (Ed.) Surface Level Ozone Exposures and Their Effects on Vegetation, Lewis Publishers, Chelsea, pp 189–260
Samuelson, LJ, Kelly, JM, Mays, PA, Edwards, GS (1996) Growth and nutrition of Quercus rubra L. seedlings and mature trees after three seasons of ozone exposure. Environ Pollut 91: 317–323
Schier, GA (1990) Response of yellow-poplar (Liriodendron tulipifera L.) seedlings to simulated acid rain and ozone—2. Effect on throughfall chemistry and nutrients in the leaves. Environ Exp Bot 30: 325–331
Schimel, JP, Gulledge, J (1998) Microbial community structure and global trace gases. Glob Chang Biol 4: 745–758
Skärby, L, Ro-Poulsen, H, Wellburn, FAM, Sheppard, LJ (1998) Impacts of ozone on forests: a European perspective. New Phytol 139: 109–122
Temple, PJ, Riechers, GH (1995) Nitrogen allocation in ponderosa pine seedlings exposed to interacting ozone and drought stresses. New Phytol 130: 97–104
Updegraff, DM (1969) Semimicro determination of cellulose in biological materials. Anal Biochem 32: 420–424
Vesterdal, L (1999) Influence of soil type on mass loss and nutrient release from decomposing foliage litter of beech and Norway spruce. Can J For Res 29: 95–105
Waldrop, MP, Firestone, MK (2004) Microbial community utilization of recalcitrant and simple carbon compounds: impact of oak-woodland plant communities. Oecologia 138: 275–284
Werner, H, Fabian, P (2002) Free-air fumigation of mature trees—a novel system for controlled ozone enrichment in grown-up beech and spruce canopies. ESPR—Environ Sci Pollut Res 9(2): 117–121
White, TJ, Bruns, T, Lee, S, Taylor, JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, MA, Gelfand, DH, Sninsky, JJ, White, TJ (Eds.) PCR Protocols: A Guide to Methods and Applications, Academic Press, New York, pp 315–322
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The study was supported by a research grant from Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany (SFB 607).
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Aneja, M.K., Sharma, S., Fleischmann, F. et al. Influence of Ozone on Litter Quality and Its Subsequent Effects on the Initial Structure of Colonizing Microbial Communities. Microb Ecol 54, 151–160 (2007). https://doi.org/10.1007/s00248-006-9183-0
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DOI: https://doi.org/10.1007/s00248-006-9183-0