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Investigation of the Acute Toxic Effect of Chlorpyrifos on Pseudomonas putida in a Sterilized Soil Environment Monitored by Microcalorimetry

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Abstract

Chlorpyrifos (CPF) is moderately persistent in soils. In our study, microcalorimetry was introduced for the first time to explore the acute toxic effect of CPF on a Pseudomonas strain in sterilized soil. Firstly, it was determined by microcalorimetry that P. putida failed to degrade CPF. Then the acute toxicity of increasing concentrations of CPF to P. putida was determined by its temporal effects on metabolism and counts of colony forming units. Results revealed that the increase of CPF concentration could induce a decrease of the growth rate constant (k) and the total thermal effect (Q T), representing an inhibiting action on P. putida. In addition, the colony forming units (CFU) for P. putida were counted. Results showed that the number of P. putida decreased with increasing CPF dose after 18 h of incubation in sterilized soil. Interestingly, the trend of the number of CFU was similar to the growth rate constant k, whereas the trend became irregular after 36 h of incubation. This indicated that P. putida resisted and also expresses high metabolic activity during the exponential growth phase of 18 h; thereafter the microorganisms showed a certain adaptation, even declining in number and activity.

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Acknowledgements

This work was supported in part by grants from National Outstanding Youth Research Foundation of China (40925010), International Joint Key Project from National Natural Science Foundation of China (40920134003), National Natural Science Foundation of China (40673065, 40873060), and International Joint Key Project from Chinese Ministry of Science and Technology (2009DFA92830), and the 111 Project (B08030).

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Authors and Affiliations

  1. Key Laboratory of Biogeology and Environmental Geology Laboratory of Chinese Ministry of Education and School of Environmental Studies and Sino-Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Huilun Chen, Jun Yao, Fei Wang, Yong Zhou, Ke Chen & Rensheng Zhuang

  2. Department of Chemical Technology and Environmental Chemistry, Eötvös University, P.O. Box 32, 1518, Budapest, Hungary

    Gyula Zaray

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  1. Huilun Chen
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  2. Jun Yao
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  3. Fei Wang
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  4. Yong Zhou
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  5. Ke Chen
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  7. Gyula Zaray
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Correspondence to Jun Yao.

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Chen, H., Yao, J., Wang, F. et al. Investigation of the Acute Toxic Effect of Chlorpyrifos on Pseudomonas putida in a Sterilized Soil Environment Monitored by Microcalorimetry. Arch Environ Contam Toxicol 58, 587–593 (2010). https://doi.org/10.1007/s00244-009-9404-x

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