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Stenotrophomonas maltophilia IMV B-7288, Pseudomonas putida IMV B-7289 and Bacillus megaterium IMV B-7287 – new selected destructors of organochlorine pesticide hexachlorocyclohexane

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Abstract

Microbial destruction of organochlorine pollutants is the one of the most effective approaches, the safest and promising methods for remediation the environment from pollution. This study presents strains of microorganisms that destroy hexachlorocyclohexane: Stenotrophomonas maltophilia IMV B-7288, Pseudomonas putida IMV B-7289 and Bacillus megaterium IMV B-7287—newly selected destructors of organochlorine pesticide hexachlorocyclohexane. Their advantages and features are considered, namely—exclusively of natural origin—microorganisms are isolated from places of total pollution. The studied strains are characterized by high resistance to the HCH contaminant (in the range of 100–1000 mg/l) and the ability to decompose in soil and liquid medium. We have found that strains B. megaterium IMV B-7287 and P. putida IMV B-7289 showed a high efficiency of destruction of HCH in laboratory studies when cultivated on a chlorine-free MM medium at 70.4–89.3% from initial content. For S. maltophilia IMV B-7288 has been found that the ability to degrade HCH-isomers depends on the season a little and it was at maximum in the summer for every studied HCH-isomer: 61.6–82.1% from initial amount. The investigated strains are promising for further work to create microbial compositions with the aim to provide an effictive destruction of HCH-isomers complex.

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Abbreviations

HCH:

Hexachlorocyclohexane

IMV:

D.K. Zabolotniy Institute of Microbiology and Virology of National Academy of Science of Ukraine

MPC:

Maximum permissible concentrations

CFU:

Colony forming units

MM:

Medium, Menkina’s mineral medium

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Acknowledgements

This study was supported by “Functional potential of soil microbiome at agrocenoses and technogenic ecotopes” State Departmental Topic (0120U000220).

Funding

This study was supported by Government scientific program “The Functional potentiality of soil microbiome at agrocenoses and technogenic ecotopes” State Government Departmental program thematic range 0120U000220 and was supported by Target program of scientific research of National Academy of Science of Ukraine “Genomic, molecular and cellular bases for development of innovative biotechnologies” (2020–2024).

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Authors and Affiliations

  1. Department of General and Soil Microbiology, D.K. Zabolotny Institute of Microbiology and Virology of National Academy of Science of Ukraine, 154, Akad. Zabolotnogo str, Kiev, 03680, Ukraine

    Galyna Iutynska & Nadiia Yamborko

  2. Engineering, Head of Scientific and Methodical Laboratory of Chromatographic Researches, Mineconomdevelopment of Ukraine SE “Ukrmetrteststandart”, 4, Metrologichna Str, Kiev, 03143, Ukraine

    Iryna Levchuk

Authors
  1. Iryna Levchuk
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  2. Galyna Iutynska
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  3. Nadiia Yamborko
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NY, GI, IL wrote the article. All authors contributed to the conception, design and critically revised the manuscript.

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Correspondence to Nadiia Yamborko.

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Communicated by Erko Stackebrandt.

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Levchuk, I., Iutynska, G. & Yamborko, N. Stenotrophomonas maltophilia IMV B-7288, Pseudomonas putida IMV B-7289 and Bacillus megaterium IMV B-7287 – new selected destructors of organochlorine pesticide hexachlorocyclohexane. Arch Microbiol 204, 611 (2022). https://doi.org/10.1007/s00203-022-03220-1

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