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Novel Chaperonins in a Prokaryote

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Abstract

Group II chaperonins belong to the Hsp60 family occurring in archaea and eukaryotes. The archaeal chaperonins build the thermosome, which is similar to the eukaryotic CCT (chaperonin-containing TCP-1). Eukaryotes have eight subunits, and up until now, it was thought that archaea had between one and three subunits, depending on the species. We now report two novel subunits, termed Hsp60-4 and Hsp60-5, in the archaeon Methanosarcina acetivorans, which also has Hsp60-1, Hsp60-2, and Hsp60-3 with orthologs in Methanosarcinae. Hsp60-4 and Hsp60-5 occur only in M. acetivorans, which makes this organism unique in that it has the highest number of chaperonin subunits ever described for an archaeon. Evolutionary analysis suggests that either Hsp60-4 or Hsp60-5 paralogs have arisen by gene duplication with vastly increased accepted substitution rates or that they represent ancestral types found only in this species.

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Acknowledgements

We are grateful to Mona Malz and Tara Garcia-Collins for their help and to the staff of the Photography and Illustration Unit of the Wadsworth Center for their expert assistance with the figures.

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Authors and Affiliations

  1. Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD, 21202, USA

    Dennis L. Maeder

  2. Wadsworth Center, New York State Department of Health, and Department of Biomedical Sciences, The University at Albany (SUNY), Albany, NY 12201, USA

    Alberto J.L. Macario & Everly Conway de Macario

  3. Wadsworth Center, Room B-749, New York State Department of Health, Everly Conway de Macario, Empire State Plaza, P.O. Box 509, , Albany, NY, 12201-0509, USA

    Everly Conway de Macario

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  1. Dennis L. Maeder
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  2. Alberto J.L. Macario
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  3. Everly Conway de Macario
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Correspondence to Everly Conway de Macario.

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Reviewing Editor: Dr. W. Ford Doolittle

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Maeder, D.L., Macario, A.J. & de Macario, E.C. Novel Chaperonins in a Prokaryote. J Mol Evol 60, 409–416 (2005). https://doi.org/10.1007/s00239-004-0173-x

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