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An In Vitro Study of the Antioxidant and Antihemolytic Properties of Buddleja globosa (Matico)

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Abstract

The antioxidant and antihemolytic properties contained in the leaves of Buddleja globosa (B. globosa), also known as “Matico,” were determined. Aqueous extracts of leaves were assayed in human erythrocytes and molecular models of its membrane. The latter were bilayers built-up of lipids located in the outer and inner leaflets of the erythrocyte membrane. Observations by scanning electron microscopy showed that the extract altered the morphology of erythrocytes inducing the formation of crenated echinocytes. This result implied that the extract components were inserted into the outer leaflet of the cell membrane. This conclusion was confirmed by experiments carried out by fluorescence spectroscopy of red cell membranes and vesicles (LUV) of dimyristoylphosphatidylcholine (DMPC) and by X-ray diffraction of DMPC and dimyristoylphosphatidylethanolamine bilayers. Human erythrocytes were in vitro exposed to HClO, which is a natural powerful oxidant. Results demonstrated that low concentrations of B. globosa aqueous extract neutralized the harmful capacity of HClO. Hemolysis experiments also showed that the extract in very low concentrations reduced hemolysis induced by HClO.

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Abbreviations

Buddleja globosa :

B. globosa

DMPC:

Dimyristoylphosphatidylcholine

DMPE:

Dimyristoylphosphatidylethanolamine

SEM:

Scanning electron microscopy

GAE:

Gallic acid equivalents

RBCS:

Red blood cell suspension

ORAC:

Oxygen radical absorbance capacity

IUM:

Isolated unsealed human erythrocytes

LUV:

Large unilamellar vesicles

GP:

Generalized polarization

r:

Fluorescence anisotropy

AAPH:

2,2′-Azobis(2-amidino-propane)dihydrochloride

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Acknowledgements

The authors thank Dr. M. Avello and Fernando Neira for their technical assistance. This work was supported by a FONDECYT Grant (1090041).

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Authors and Affiliations

  1. Faculty of Chemical Sciences, University of Concepción, Concepción, Chile

    Mario Suwalsky

  2. Centre of Biotechnology and Bioengineering, University of Chile, Santiago, Chile

    José Duguet

  3. Nutrition and Food Technology Institute, University of Chile, Santiago, Chile

    Hernán Speisky

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  1. Mario Suwalsky
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  2. José Duguet
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Correspondence to Mario Suwalsky.

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Suwalsky, M., Duguet, J. & Speisky, H. An In Vitro Study of the Antioxidant and Antihemolytic Properties of Buddleja globosa (Matico). J Membrane Biol 250, 239–248 (2017). https://doi.org/10.1007/s00232-017-9955-0

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