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Discovery of superior bioactive peptides of two edible Lentinus mushrooms protein hydrolysate in biological activities: tyrosinase inhibitory and antioxidant activity

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Abstract

This study investigated protein hydrolysates obtained from Lentinus squarrosulus and Lentinus edodes fruiting bodies via gastric protease hydrolysis and ultrafiltration, yielding peptides with a molecular weight below 6.5 kDa. These hydrolysates displayed significant tyrosinase inhibitory activity similar to positive controls, peptide from Chinese quince seed (RHAKF) and kojic acid. L. squarrosulus-derived hydrolysates exhibited superior antioxidant properties compared to L. edodes in DPPH (47% vs. 23%) and ABTS (77% vs. 23%) assays. Identified bioactive peptides, particularly LILGGSSS from L. squarrosulus, interacted with tyrosinase through hydrogen bonds at specific residues. Notably, these protein hydrolysates showcased potent tyrosinase inhibition without cytotoxic effects, presenting promising prospects for addressing hyperpigmentation caused by excessive tyrosinase activity from stress or UV exposure.

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Acknowledgements

This work was supported by NU Fundamental Fund 2565 (Project Code #2335267) and NRCT graduated students (2564/3 and 2564/5). Docking software was kindly supported by Assoc.Prof.Dr. Kiattawee Choowongkomon.

Funding

Funding was provided by National Research Council of Thailand (Grant No. 2564/3), Thailand Science Research and Innovation (Grant No. 2335267) and National Research Council of Thailand (Grant No. 2564/5).

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, Thailand

    Worawut Kriangkrai

  2. Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand

    Tattiya Kantasa, Wannaporn Sagasae, Chaturong Inpad, Worasak Kaewkong & Jongrak Attarat

  3. Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand

    Sittiruk Roytrakul

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  1. Worawut Kriangkrai
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Kriangkrai, W., Kantasa, T., Sagasae, W. et al. Discovery of superior bioactive peptides of two edible Lentinus mushrooms protein hydrolysate in biological activities: tyrosinase inhibitory and antioxidant activity. Food Sci Biotechnol (2024). https://doi.org/10.1007/s10068-024-01563-7

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