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Separation and purification of angiotensin-I-converting enzyme (ACE) inhibitory peptides from walnuts (Juglans regia L.) meal

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Abstract

Angiotensin-I-converting enzyme (ACE) inhibitory peptides were isolated from walnut pepsin hydrolysates using ultrafiltration, Sephadex G-25 chromatography, reverse-phase high-performance liquid chromatography (RP-HPLC), and the ÄKTA preparative HPLC separation and purification system. ACE inhibitory activity was measured using in vitro HPLC analysis, and N-terminal amino acid sequencing was used for structural identification. Those fractions with the highest ACE inhibitory activity were further purified using RP-HPLC and the ÄKTA systems. The most active ACE inhibitory peptide had the sequence Tyr-Val-Pro-His-Trp-Asp-Leu and a molecular weight of 929 Da. Its activity, along with the activity of a synthetic ACE inhibitory peptide with the same sequence, was measured using HPLC following in vitro gastrointestinal digestion experiments. The IC50 values obtained before and after digestion were 0.136 and 0.173 μM/mL, respectively, which indicated that the peptide was relative stable during digestion and might have in vivo biological activity.

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References

  1. Wu WL, Wu GJ, Liang DS, Yang F (2006) The physiological function and research progress of angiotensin-I-converting enzyme inhibitory peptides. Mod Food Sci Technol 22:251–254

    Google Scholar 

  2. Zhao YH, Gong JJ, Li ZH, Tang J (2011) Research progress of ACE inhibitory peptide. Cereals Oils 25:44–46

    Google Scholar 

  3. Ferreira SH (1965) A bradykinin-potentiating factor (BPF) present in the venom of Bothrops jararaca. Br J Pharmacol Chemother 24:163–169

    Article  CAS  Google Scholar 

  4. Majumder K, Wu JP (2011) Purification and characterisation of angiotensin I converting enzyme (ACE) inhibitory peptides derived from enzymatic hydrolysate of ovotransferrin. Food Chem 126:1614–1619

    Article  CAS  Google Scholar 

  5. Hao W, He HL, Chen XL, Sun CY, Zhang YZ, Zhou BC (2008) Purification and identification of novel angiotensin-I-converting enzyme inhibitory peptides from shark meat hydrolysate. Process Biochem 43:457–461

    Article  Google Scholar 

  6. Gu RZ, Li CY, Liu WY, Yi WX, Cai MY (2011) Angiotensin I-converting enzyme inhibitory activity of low-molecular-weight peptides from Atlantic salmon (Salmo salar L.) skin. Food Res Int 44:1536–1540

    Article  CAS  Google Scholar 

  7. Vercruysse L, Van Camp J, Smagghe G (2005) ACE inhibitory peptides derived from enzymatic hydrolysates of animal muscle protein: a review. J Agric Food Chem 53:8106–8115

    Article  CAS  Google Scholar 

  8. Lu J, Ren DF, Wang JZ (2010) Purification and characterization of an angiotensin I-converting enzyme inhibitory peptide derived from Spirulina platensis. Progress Biochem Biophys 37:568–574

    Article  CAS  Google Scholar 

  9. Hernández-Ledesma B, María del Mar C, Recio I (2011) Antihypertensive peptides: production, bioavailability and incorporation into foods. Adv Colloid Interface Sci 165:23–35

    Article  Google Scholar 

  10. Samarakoon KW, O-Nam K, Ko JY, Lee JH, Kang MC, Kim D, Lee JB, Lee JS, Jeon YJ (2013) Purification and identification of novel angiotensin-I converting enzyme (ACE) inhibitory peptides from cultured marine microalgae (Nannochloropsis oculata) protein hydrolysate. J Appl Phycol 25:1595–1606

    Article  CAS  Google Scholar 

  11. Ko SC, Kang N, Kim EA, Kang MC, Lee SH, Kang SM (2012) A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from a marine Chlorella ellipsoidea and its antihypertensive effect in spontaneously hypertensive rats. Process Biochem 47:2005–2011

    Article  CAS  Google Scholar 

  12. Liu Y, Lv X, Zhang ZX (2008) Hypotensive effect and mechanism of lactic acid bacteria and fermentation products. Inn Mong Sci Technol Econ 12:71–72

    Google Scholar 

  13. Jakubczyk A, Baraniak B (2013) Activities and sequences of the angiotensin I-converting enzyme (ACE) inhibitory peptides obtained from the digested lentil (Lens culinaris) globulins. Int J Food Sci Technol 48:2363–2369

    CAS  Google Scholar 

  14. Wang S, Wang CT, Han Y (2010) Separation, purification and activity of ace inhibitory peptides from oat. Food Sci 31:222–229

    Google Scholar 

  15. Zhou HJ, Zhu ZB, Yi JH (2013) Purification of ACE inhibitory peptides from enzymatic hydrolysate of walnut protein. Cereals Oils 27:16–18

    CAS  Google Scholar 

  16. Jao CL, Huang SL, Hsu KC (2012) Angiotensin I-converting enzyme inhibitory peptides: inhibition mode, bioavailability, and antihypertensive effects. Bio Med 2:130–136

    Google Scholar 

  17. Mao XY, Hua YF (2014) Chemical composition, molecular weight distribution, secondary structure and effect of NaCl on functional properties of walnut (Juglans regia L.) protein isolates and concentrates. J Food Sci Technol 51:1473–1482

    Article  CAS  Google Scholar 

  18. Labuckas D, Maestri D, Lamarque A (2014) Effect of different oil extraction methods on proximate composition and protein characteristics of walnut (Juglans regia L.) flour. LWT Food Sci Technol 59:794–799

    Article  CAS  Google Scholar 

  19. Chen N, Yang HM, Sun Y, Niu J, Liu SY (2012) Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates. Peptides 38:344–349

    Article  CAS  Google Scholar 

  20. Gu X, Li D, Hou YK, Cui J, Wang JZ (2013) Separation and purification of angiotensin I-converting enzyme inhibitor from walnut protein. Food Sci 34:53–55

    Google Scholar 

  21. Gu X, Hou YK, Li D, Wang JZ, Wang FJ (2015) Separation, purification, and identification of angiotensin I-converting enzyme inhibitory peptides from walnut (Juglans regia L.) hydrolyzate. Int J Food Prop 18:266–276

    Article  CAS  Google Scholar 

  22. Se-Kwon K, Hee-Guk B, Pyo-Jam P, Fereidoon S (2001) Angiotensin I converting enzyme inhibitory peptides purified from bovine skin gelatin hydrolysate. J Agric Food Chem 49:2992–2997

    Article  Google Scholar 

  23. Li H, Liu TX (2007) Simulated digestion of hemoglobin-rich peptides absorbed evaluation. Sci Technol Food Ind 29:207–209

    Google Scholar 

  24. Yu YD, Yu ZP, Zhao WZ, Liu JB (2010) Study on stability of angiotensin converting enzyme inhibitory peptides. Sci Technol Food Ind 32:412–414

    CAS  Google Scholar 

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Acknowledgments

This work was supported by the “Fundamental Research Funds for the Central University (No. TD2012-03) (No. 2015ZCQ-SW-04)” and the “Beijing Agricultural Technology Project (No. 20130110).” The authors would like to thank Hebei Jingpin Fruit Industry Company for providing the walnut meal and the Beijing Xinongsheng Biotechnology Co., Ltd. for the work on the separation using the ÄKTA system.

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Authors and Affiliations

  1. Department of Food Science, College of Biological Science and Technology, Beijing Forestry University, Post Box 112, No. 35 Qinghua East Rd, Haidian District, Beijing, 100083, China

    Feng-Jun Wang, Xiao-Yan Yin & Jian-Zhong Wang

  2. Department of Food Science, Cornell University, Ithaca, NY, 14853-7201, USA

    Joe M. Regenstein

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  1. Feng-Jun Wang
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  2. Xiao-Yan Yin
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  4. Jian-Zhong Wang
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Correspondence to Feng-Jun Wang.

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Wang, FJ., Yin, XY., Regenstein, J.M. et al. Separation and purification of angiotensin-I-converting enzyme (ACE) inhibitory peptides from walnuts (Juglans regia L.) meal. Eur Food Res Technol 242, 911–918 (2016). https://doi.org/10.1007/s00217-015-2597-5

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  • DOI: https://doi.org/10.1007/s00217-015-2597-5

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