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Multi-microbial metabolites in fonio millet (acha) and sesame seeds in Plateau State, Nigeria

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Abstract

We investigated 16 fonio millet and 17 sesame samples by LC/ESI–MS/MS for the spectrum of microbial metabolites contaminating these seeds. Forty-eight fungal and four bacterial metabolites were detected in fonio, while 28 fungal and two bacterial metabolites were found in sesame. Altogether, 55 metabolites were identified in both grains, 18 of which are reported for the first time to naturally occur in cereals and oil seeds. The metabolite concentrations reached 7,280 μg/kg in fonio for aurofusarin and 64,600 μg/kg in sesame for kojic acid. Aflatoxin contaminated 81 % of fonio samples at concentrations less than the 4 μg/kg maximum allowable limit (MAL) set by European Union (EU). In contrast, aflatoxin was not detected in sesame. Zearalenone levels exceeded the EU MAL (75 μg/kg) in one sample of fonio (987 μg/kg). About 62.5 % (30 out of 48) of the metabolites without regulations occurred in more than 50 % of samples of one or both seeds, while 3-nitropropionic acid, beauvericin, brevianamid F, curvularin, emodin, equisetin, macrosporin A, monocerin and tenuazonic acid were the most prevalent, occurring in all samples of either fonio, sesame or both. This is the first study reporting mycotoxin contamination in sesame in Nigeria and the broad range of microbial metabolites in millet and sesame.

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Acknowledgments

The authors are thankful to Iyeneh E. Udom for assisting in sample collection and Ezinne Aja for careful linguistic revision.

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. C. N. Ezekiel

    Present address: Pathology/Mycotoxin Laboratory, International Institute of Tropical Agriculture, PMB 5320, Ibadan, Nigeria

Authors and Affiliations

  1. Mycology/Mycotoxicology Research Unit, Department of Biosciences and Biotechnology, Babcock University, Ilishan Remo, Ogun State, Nigeria

    C. N. Ezekiel

  2. Department of Agrobiotechnology (IFA-Tulln), Center for Analytical Chemistry, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenzstr. 20, 3430, Tulln, Austria

    M. Sulyok, B. Warth & R. Krska

Authors
  1. C. N. Ezekiel
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  2. M. Sulyok
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  3. B. Warth
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  4. R. Krska
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Correspondence to C. N. Ezekiel.

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Ezekiel, C.N., Sulyok, M., Warth, B. et al. Multi-microbial metabolites in fonio millet (acha) and sesame seeds in Plateau State, Nigeria. Eur Food Res Technol 235, 285–293 (2012). https://doi.org/10.1007/s00217-012-1755-2

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  • DOI: https://doi.org/10.1007/s00217-012-1755-2

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