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Preparation of water-soluble chitosan by hydrolysis with commercial glucoamylase containing chitosanase activity

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Abstract

Water-soluble chitosan (WSC) was prepared by hydrolyzing chitosan with commercial glucoamylase. Factors affecting the enzymatic hydrolysis of chitosan were investigated, and the optimum hydrolysis conditions were as follows: time, 24 h; pH, 4.5; temperature, 55 °C; amount of glucoamylase, 40 mg/500 mL of reaction mixture (26.8 U of chitosanase activity). Under these conditions, the weight-averaged molecular weight (Mw) of chitosan decreased to 1,700 Da. The hydrolysate was neutralized with 1 M NaOH, filtrated, concentrated to 16% (w/v), and precipitated with 5 volumes of ethanol. The product of WSC was obtained by drying the precipitate at 60 °C for 3 h. The content of WSC in the product and the yield of WSC were 95.3% (w/w) and 94.2% (w/w), respectively. The structure of WSC was characterized by Fourier transform infrared spectroscopy (FTIR).

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  1. School of Marine Science and Technology, HuaiHai Institute of Technology, 59 Cangwu Road, Lianyungang, 222005, China

    Saikun Pan & Shengjun Wu

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  1. Saikun Pan
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  2. Shengjun Wu
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Correspondence to Shengjun Wu.

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Pan, S., Wu, S. Preparation of water-soluble chitosan by hydrolysis with commercial glucoamylase containing chitosanase activity. Eur Food Res Technol 233, 325–329 (2011). https://doi.org/10.1007/s00217-011-1524-7

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  • DOI: https://doi.org/10.1007/s00217-011-1524-7

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