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Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events

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Abstract

Specific legislation in the EU requires that foods containing more than 0.9% of genetically modified organisms (GMOs) should be labelled. To this end, we have developed a robust, quantitative, sensitive, nine-plex ligation-dependent probe amplification method, GMO-MLPA, for event-specific detection of maize TC1507, MON810, NK603, MON863, BT176, T25, GA21, construct-specific detection of BT11, and detection of the endogenous hmga maize reference gene. Ligated probes are amplified by PCR. Amplicons are detected using capillary electrophoresis. Specific GMO signals are normalised relative to the signal from the endogenous hmga gene and quantified by comparing with known standard curves. The method is suitable for quantification in the 0–2% range. Agreement was obtained in 149 of 160 determinations when 11 known mixtures of GMO and 9 food and feed samples were analysed using the GMO-MLPA method and compared to results from quantitative real-time 5′-nuclease PCR. The presented method is, therefore, suitable for quantification purposes for food and feed containing the most common maize GMOs.

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Acknowledgments

This work was financially supported by the European Commission through the Sixth Framework Program, integrated project Co-Extra (http://www.coextra.eu; contract FOOD-2005-CT-007158) and Norwegian Research Council project 154254/130.

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Authors and Affiliations

  1. Nofima Mat, Osloveien 1, 1430, Aas, Norway

    Askild Lorentz Holck, Signe M. Drømtorp & Even Heir

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  1. Askild Lorentz Holck
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  2. Signe M. Drømtorp
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  3. Even Heir
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Correspondence to Askild Lorentz Holck.

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Holck, A.L., Drømtorp, S.M. & Heir, E. Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events. Eur Food Res Technol 230, 185–194 (2009). https://doi.org/10.1007/s00217-009-1155-4

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  • DOI: https://doi.org/10.1007/s00217-009-1155-4

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