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Authentication of three valuable Dendrobium species by adapter ligation-mediated allele-specific amplification

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Abstract

The adapter ligation-mediated allele-specific amplification (ALM-ASA) has been applied to authenticate three valuable Dendrobium species (Dendrobium aphyllum, Dendrobium devonianum and Dendrobium officinale) and their corresponding “Fengdou” foods samples. Three species-specific primers and a universal primer were designed. By using these primers, three amplification products are 198 bp (D. aphyllum), 250 bp (D. devonianum) and 310 bp (D. officinale) in length, which were clearly distinguishable on a 2.0% agarose gel. In addition, no band was observed with other 17 allied Dendrobium species of “Fengdou”. The results showed that ALM-ASA could be used to authenticate not only the three valuable Dendrobium species but related food products such as “Fengdou” foods by simultaneous detection of three-site nucleotide difference on rDNA internal transcribed spacer region.

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Acknowledgments

We would like to thank the National Natural Science Foundation of China & Natural Science Foundation of Jiangsu Province for financial support (No. 30370144, No. 30870234, No. BK2008431).

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Authors and Affiliations

  1. Jiangsu Provincial Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210046, China

    Weichao Zhang, Xiaoyu Ding, Minglu Xie, Zhenyu Feng, Shan Lu, Xuexia Li, Feng Zhang & Ge Ding

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  1. Weichao Zhang
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  2. Xiaoyu Ding
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  3. Minglu Xie
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  4. Zhenyu Feng
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  5. Shan Lu
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  6. Xuexia Li
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  7. Feng Zhang
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  8. Ge Ding
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Correspondence to Xiaoyu Ding.

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Zhang, W., Ding, X., Xie, M. et al. Authentication of three valuable Dendrobium species by adapter ligation-mediated allele-specific amplification. Eur Food Res Technol 229, 1–7 (2009). https://doi.org/10.1007/s00217-009-1019-y

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  • DOI: https://doi.org/10.1007/s00217-009-1019-y

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