Abstract
The lactic acid bacteria (LAB) of southern Caucasus region present a special interest due to the diversity of lactic flora used for fermentations by various local populations during thousands of years. Four LAB strains, not identified previously, isolated from Motal and Brunza typical Azerbaijani cheeses were subjected to phenotypic identification and three of them could be identified as Lactobacillus paracasei subsp. paracasei and one as Lactobacillus rhamnosus. Test strains such as Lactobacillus bulgaricus 340 and Saccharomyces cerevisiae were inhibited by the four isolated strains. Antibacterial activity against Escherichia coli HB 101 was detected in Lactobacillus paracasei BN ATS 8w and L. rhamnosus FAZ 16m. L. paracasei BN ATS 5w and 8w, and L. rhamnosus FAZ 16m showed inhibitory effect on Staphylococcus aureus Cip 9973. The inhibition of Candida pseudotropicalis was detected only when using L. paracasei species BN ATS 5w and 7w. Culture of Listeria innocua was insensitive to the antimicrobial substances of all the studied strains. Complete inactivation or significant reduction in antibacterial activity was observed after treatment of cell-free supernatants with pronase E and proteinase K, but not with trypsin (except for L. rhamnosus FAZ 16m), indicating the protein nature of the active agents. Amylase treatment totally inactivated the substances of L. paracasei, what implies the importance of glycosylation for the activity. The activities of all the bacteriocin-like substances from studied LABs were stable over a wide pH range from 3 to 11.
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Daeschel MA (1989) Food Technol 43:164–167
De Vuyst L, Vandamme EJ (1994) In: de Vuyst L, Vandamme EJ (eds) Bacteriocins of lactic acid bacteria: microbiology, genetics and applications. Blackie Academic and Professional, London, pp 91–142
De Vuyst L, Vandamme EJ (1994) In: de Vuyst L, Vandamme EJ (eds) Bacteriocins of lactic acid bacteria: microbiology, genetics and applications. Blackie Academic and Professional, London, pp 1–12
Schillinger U (1990) In: Bills DD, Kung S (eds) Biotechnology and food safety. Butterworth-Heinemann, Boston, pp 55–74
Atrih A, Rekhif N, Moir AJG, Lebrihi A, Lefebvre G (2001) Int J Food Microbiol 68:93–104
Messens W, De Vuyst L (2002) Int J Food Microbiol 72:31–43
Corsetti A, Gobbetti M, Rossi J, Damiani P (1998) Appl Microbiol Biot 50:253–256
Lavermicocca P, Valerio F, Evidente A, Lazzaroni S, Corsetti A, Gobbetti M (2000) Appl Environ Microb 66:4084–4090
Schillinger U, Holzapfel WH (1996) Int J Food Microbiol 33:3–5
Nes IF, Diep BD, Havarstein LS, Brurberg MB, Eijsink V, Holo H (1996) Anton Leeuw Int J G 70:113–128
Klaenhammer TR (1993) FEMS Microbiol Rev 12:39–86
Cleveland J, Montville TJ, Nes IF, Chikindas ML (2001) Int J Food Microbiol 71:1–20
Ross RP, Morgan S, Hill C (2002) Int J Food Microbiol 79:3–16
Nes IF, Holo H (2000) Biopolymers 55:50–61
Ferchichi M, Frere J, Mabrouk K, Manai M (2001) FEMS Microbiol Lett 205:49–55
Simon L, Fremaux C, Cenatiempo Y, Berjeaud JM (2002) Appl Environ Microb 68:6416–6420
Muriana PM, Klaenhammer TR (1991) Appl Environ Microb 57:114–121
Joerger MC, Klaenhammer TR (1990) J Bacteriol 172:6339–6347
Ghrairi T, Manai M, Berjeaud JM, Frere J (2004) J Appl Microbiol 97:621–628
Vaughan EE, Daly C, Fitzgerald GF (1992) J Appl Bacteriol 73:299–308
Thompson JK, Collins MA, Mercer WD (1996) J Appl Bacteriol 80:338–348
Elotmani F, Revol-Junelles AM, Assobhei O, Milliere JB (2002) Curr Microbiol 44:10–17
Tagg JR, McGiven AR (1971) Appl Microbiol 21:943
Gonzales B, Kunka J (1987) Appl Environ Microb 49:627–633
Schillinger U, Lucke FK (1989) Appl Environ Microb 55:1901–1906
Skytta E, Hereijgers H, Mattila-Sandholm W (1991) Food Microbiol 8:231–237
Toba T, Samant SK, Yoshioka E, Itoh T (1991) Lett Appl Microbiol 13:281–286
Garver KI, Muriana M (1993) Int J Food Microbiol 19:241–258
Valdés-Stauber N, Götz H, Busse M (1991) Int J Food Microbiol 13:119–130
Ryser ET, Maisnier-Patin S, Gratadoux JJ, Richard J (1994) Int J Food Microbiol 21:237–246
Coventry MJ, Gordon JB, Wilcock A, Harmark K, Davidson BE, Hickey MW, Hillier AJ, Wan J (1997) J Appl Microbiol 83:248–258
Tagg JR, Dajani AS, Wannamaker LW (1976) Bacteriol Rev 40:722–756
Jack RW, Tagg JR, Ray B (1995) Microbiol Rev 59:171–200
Lewus CB, Sun S, Montville JT (1992) Appl Environ Microbiol 58:143–149.
Keppler K, Geiser R, Holzapfel WH (1994) Food Microbiol 11:39–45
Losteinkit C, Uchiyama K, Ochi S, Takaoka T, Nagahisa K, Shioya S (2001) J Biosci Eng 91:390–395
Atanassova M, Choiset Y, Dalgalarrondo M, Chobert J-M, Dousset X, Ivanova I, Haertlé T (2003) Int J Food Microbiol 87:63–73
Boris S, Barbes C (2000) Microbes Infect 2:543–546
McGroatry JA (1993) FEMS Immunol Med Mic 61:251–264
Piard JC, Delorme F, Giraffa G, Commissaire J, Desmazeaud M (1990) Neth Milk Dairy J 44:143–158
Lewus C, Kaiser A, Montville T (1991) Appl Environ Microb 57:1683–1688
Kojic M, Svircevic J, Banina A, Topisirovic L (1991) Appl Environ Microb 6:1835–1837
Gonzales B, Arca P, Mayo B, Suarez J (1994) Appl Environ Microb 6:2158–2163
Ivanova I, Miteva V, Stefanova T, Pantev A, Budakov I, Danova S, Moncheva P, Nikolova I, Dousset X, Boyaval P (1998) Int J Food Microbiol 42:147–158
De Klerk HC, Smit JA (1967) J Gen Microbiol 48:309–316
Upretti GC, Hinsdill RD (1973) Antimicrob Agents Ch 4:487–497
Piard J, Desmazeaud M (1992) Lait 72:113–142
Jimenez-Diaz R, Ruiz-Barba JL, Cathcart DP, Holo H, Nes IF, Sletten KH, Warner PJ (1995) Appl Environ Microb 61:4459–4463
Suma K, Misra MC, Varadaraj MC (1998) Int J Food Microbiol 40:17–25
Corsetti A, Gobbetti M (2002) In: Rodinki H, Fuguai JW, Fox PF (eds) Encyclopedia of dairy sciences, Academic Press, San Diego, CA, vol 3, pp 1501–1507
Schneusner DL, Hood LL, Harmon LG (1973) J Milk Food Technol 36:249–252
Smith JL, Buchanan RL, Palumbo SA (1983) J Food Protect 46:545–555
Lozo J, Vukasinovic M, Strahinic I, Topisirovic L (2004) J Food Protect 67:2727–2734
Cardi A (2004) J Ind Microbiol Biot 29:303–308
Sameshima TC, Magome K, Takeshita K, Arihara M, Itoh M, Kondo Y (1998) Int J Food Microbiol 41:1–7
Ennahar S, Aoude-Werner D, Sorokine O, van Dorsselaer A, Bringel F, Hubert JC, Hasselmann C (1996) Appl Environ Microb 62:4381–4387
Rodriguez E, Gonzales B, Gaya P, Nunez M, Medina M (2000) Int Dairy J 10:7–15
Navarro L, Zarazaga M, Saenz J, Ruiz-Larrea F, Torres C (2000) J Appl Microbiol 88:44–51
Acknowledgements
This study has been supported by an ECO-NET grant no. 08139XA and by the grant of French Embassy in Azerbaijan Republic. The authors express their gratitude to Dr. Hugues Baltzinger and to French Ministry of Foreign Affaires for a grant from the French Embassy.
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Gurban oglu Gulahmadov, S., Batdorj, B., Dalgalarrondo, M. et al. Characterization of bacteriocin-like inhibitory substances (BLIS) from lactic acid bacteria isolated from traditional Azerbaijani cheeses. Eur Food Res Technol 224, 229–235 (2006). https://doi.org/10.1007/s00217-006-0338-5
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DOI: https://doi.org/10.1007/s00217-006-0338-5