Abstract
Response surface methodology was used to study the effect of pressure (0–600 MPa) and time (0–1,800 s) on the surface hydrophobicity, reactive sulphydryl groups content and the flowing properties of bovine myofibrillar proteins in solution at 10 g/l. Results show that high-pressure treatment induced a threefold increase of the surface hydrophobicity of myofibrillar proteins between 0 MPa and 450 MPa. The same upward trend was obtained on the reactive sulphydryl groups, which climbed from 40 to 69%. Concerning rheological properties of solutions, the flow index tended towards a maximum value close to the Newtonian behaviour (n=1), whereas the viscosity coefficient decreased with the increase of pressure. The statistical analysis of responses proves that pressure and its quadratic effect are the most important significant effects in comparison with the effects of time. Changes in myofibrillar proteins interactions and rheological properties are related to a structure change of the proteins, and could induce better functional properties.
Similar content being viewed by others
References
Bridgman PW (1914) J Biol Chem 19:511–512
Hayashi R, Kawamura Y, Nakasa T, Okinaka O (1989) Agric Biol Chem 53:2935–2939
Famelart M-H, Chapron L, Piot M, Brule G, Durier C (1998) J Food Eng 36:149–164
Van Camp J, Huyghebaert A (1995) Lebensm-Wiss Technol 28:111–117
Nagashima Y, Ebina H, Tanaka M, Taguchi T (1993) Food Res Int 26:119–123
Sareevoravikul R, Simpson BK, Ramaswany HS (1996) J Aquat Food Prod Technol 5:65–79
Ko W-C (1996) Fish Sci 62:101–104
Perez-Mateos M, Lourenço H, Montero P, Borderias AJ (1997) J Agric Food Chem 45:44–49
Fernandez-Martin F, Perez-Mateos M, Montero P (1998) J Agric Food Chem 46:3257–3264
Angsupanich K, Ledward DA (1998) Food Chem 63:39–50
Ikeuchi Y, Tanji H, Kim K, Suzuki A (1992) J Agric Food Chem 40:1751–1755
Ikeuchi Y, Tanji H, Kim K, Suzuki A (1992) J Agric Food Chem 40:1759–1761
Yamamoto K, Hayashi S, Yasui T (1993) Bioscie Biotechnol Biochem 57:383–389
Galazka VB, Dickinson E, Ledward D (2001) Innov Food Sci Emerging Technol 1:177–185
Offer G, Moos C, Starr R (1973) J Mol Biol 74:653–676
Gornall AG, Bardawill CJ, David MM (1949) J Biol Chem 177:751–766
Kato A, Nakai S (1980) Biochem Biophys 634:13–80
Ellman GL (1958) Arch Biochem Biophys 74:443–450
Cochran WG, Cox GM (1992) Experimental designs. Wiley, New York
Derringer G, Suich R (1980) J Qual Technol 12:214–219
Hayakawa I, Linko Y-Y, Linko P (1996) Lebensm-Wiss Technol 29:756–762
O'Shea JM, Horgan DJ, Macfarlane JJ (1976) Aust J Biol Sci 29:197–207
Perry SV (1967) Progress Biophys Mol Biol 17:325–381
Hofmann K, Hamm R (1978) Adv Food Res 24:1-111
Pauling L (1960) Chemical bond. In: Pauling, L (ed) The nature of the chemical bond, 3rd edn. Cornell University Press, New York p 85
Morild E (1981) AdvProtein Chem 34:93–166
Perry SV, Cotterill J (1965) Nature (London) 206:161–163
Sano T, Noguchi J, Matsumoto J, Tsuchiya T (1989) J Food Sci 54:800–804
Acknowledgements
The region Pays de Loire supported this work. Authors thank LEIMA (INRA-Nantes) and Sylviane Delépine.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chapleau, N.J., de Lamballerie-Anton, M.I. Changes in myofibrillar proteins interactions and rheological properties induced by high-pressure processing. Eur Food Res Technol 216, 470–476 (2003). https://doi.org/10.1007/s00217-003-0684-5
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00217-003-0684-5