Abstract
The uptake of proteins is highly recommended, mainly for athletes, elderly people and patients with serious diseases like dysphagia. In this work, whey proteins were used for producing two kinds of aqueous materials: suspensions of proteins, in the native form, and gels, obtained by protein denaturation. In the first system, proteins are used as interfacial active ingredients or as thickening agents of the aqueous phase, whereas in the second one they are used as structuring agents. These protein-based materials show very different rheological and microstructural behaviour even at the same concentration. In the case of an undenatured system, a growing protein fraction resulted in an increased dimension of their aggregates and all investigated systems exhibited a liquid-like behaviour with a viscosity independent of shear rate and well described by a Krieger-Dougherty model. In the case of thermally denatured systems, it has been observed that, at increasing protein content, aggregates evolve towards a continuous gel network with a fractal behaviour. Both systems have been modelled, according to their specific behaviour, with the aim of proposing equations suitable to relate macroscopic properties and microstructure, useful for designing new food products with predictable properties for different industrial uses.
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References
Abaee A, Mohammadian M, Jafari SM (2017) Whey and soy protein-based hydrogels and nano-hydrogels as bioactive delivery systems. Trends Food Sci Technol 70:69–81. https://doi.org/10.1016/J.TIFS.2017.10.011
Andoyo R, Dianti Lestari V, Mardawati E, Nurhadi B (2018) Fractal dimension analysis of texture formation of whey protein-based foods. Int J Food Sci 2018:1–17. https://doi.org/10.1155/2018/7673259
Baldino N, Gabriele D, Migliori M (2010) The influence of formulation and cooling rate on the rheological properties of chocolate. Eur Food Res Technol 231:821–828. https://doi.org/10.1007/s00217-010-1334-3
Barnes HA, Hutton JF, Walters K (1989) An introduction to rheology, 1st edn. Elsevier Science Publishers B.V, Amsterdam
Carvalho-Silva L, Vissotto F, Amaya-Farfan J (2013) Physico-chemical properties of milk whey protein agglomerates for use in oral nutritional therapy. Food Nutr Sci 4:69–78. https://doi.org/10.4236/fns.2013.49A2010
Chevallier M, Riaublanc A, Lopez C et al (2018) Increasing the heat stability of whey protein-rich emulsions by combining the functional role of WPM and caseins. Food Hydrocoll 76:164–172. https://doi.org/10.1016/J.FOODHYD.2016.12.014
Curcio S, Gabriele D, Giordano V, Calabrò V, de Cindio B, Iorio G (2001) A rheological approach to the study of concentrated milk clotting. Rheol Acta 40:154–161. https://doi.org/10.1007/s003970000146
Dahbi L, Alexander M, Trappe V, Dhont JK, Schurtenberger P (2010) Rheology and structural arrest of casein suspensions. J Colloid Interface Sci 342:564–570. https://doi.org/10.1016/J.JCIS.2009.10.042
do Carmo WP, Lenzi MK, Lenzi EK et al (2019) A fractional model to relative viscosity prediction of water-in-crude oil emulsions. J Pet Sci Eng 172:493–501. https://doi.org/10.1016/J.PETROL.2018.09.076
Eissa AS (2013) Newtonian viscosity behavior of dilute solutions of polymerized whey proteins. Would viscosity measurements reveal more detailed molecular properties? Food Hydrocoll 30:200–205. https://doi.org/10.1016/J.FOODHYD.2012.05.018
Eissa AS, Mohamed DM, Uoness KS et al (2014) Characterization of rheological and molecular properties of whey protein thickeners. Int J Food Prop 17:570–586. https://doi.org/10.1080/10942912.2011.642445
Fabian H, Mäntele W (2001) Infrared spectroscopy of proteins. In: Chalmers M, Griffiths PR (eds) Handbook of Vibrational Spectroscopy
Gabriele D, de Cindio B, D’Antona P (2001) A weak gel model for foods. Rheol Acta 40:120–127. https://doi.org/10.1007/s003970000139
Genovese DB (2012) Shear rheology of hard-sphere, dispersed, and aggregated suspensions, and filler-matrix composites. Adv Colloid Interf Sci 171–172:1–16. https://doi.org/10.1016/j.cis.2011.12.005
Gosal WS, Ross-Murphy SB (2000) Globular protein gelation. Curr Opin Colloid Interface Sci 5(3–4):188–194
Hoffman JR, Falvo MJ (2004) Protein - which is best? J Sports Sci Med 03:118–130
Jiang S, Altaf hussain M, Cheng J et al (2018) Effect of heat treatment on physicochemical and emulsifying properties of polymerized whey protein concentrate and polymerized whey protein isolate. LWT Food Sci Technol 98:134–140. https://doi.org/10.1016/j.lwt.2018.08.028
Kharlamova A, Chassenieux C, Nicolai T (2018a) Acid-induced gelation of whey protein aggregates: kinetics, gel structure and rheological properties. Food Hydrocoll 81:263–272. https://doi.org/10.1016/J.FOODHYD.2018.02.043
Kharlamova A, Nicolai T, Chassenieux C (2018b) Calcium-induced gelation of whey protein aggregates: kinetics, structure and rheological properties. Food Hydrocoll 79:145–157. https://doi.org/10.1016/J.FOODHYD.2017.11.049
Lapasin R, Grassi M, Pricl S (1996) Rheological modeling of fractal and dense suspensions. Chem Eng J Biochem Eng J 64:99–106. https://doi.org/10.1016/S0923-0467(96)03107-7
Lapasin R, Pricl S (1995) Rheology of industrial polysaccharides: theory and applications. Blackie Academic & Professional, Glasgow
Lara BRB, Araújo ACMA, Dias MV et al (2019) Morphological, mechanical and physical properties of new whey protein isolate/ polyvinyl alcohol blends for food flexible packaging. Food Packag Shelf Life 19:16–23. https://doi.org/10.1016/J.FPSL.2018.11.010
Lupi FR, Gabriele D, Greco V et al (2013) A rheological characterisation of an olive oil/fatty alcohols organogel. Food Res Int 51:510–517. https://doi.org/10.1016/j.foodres.2013.01.013
Lupi FR, Gabriele D, Seta L et al (2014) Rheological design of stabilized meat sauces for industrial uses. Eur J Lipid Sci Technol 116:1734–1744. https://doi.org/10.1002/ejlt.201400286
Lupi FR, Gabriele D, Seta L, Baldino N, de Cindio B, Marino R (2015) Rheological investigation of pectin-based emulsion gels for pharmaceutical and cosmetic uses. Rheol Acta 54:41–52. https://doi.org/10.1007/s00397-014-0809-8
Macosko CW (1994) Rheology, principles, measurements and applications. VCH Publishers, Inc., New York
Marangoni AG, Acevedo N, Maleky F et al (2012) Structure and functionality of edible fats. Soft Matter 8:1275. https://doi.org/10.1039/c1sm06234d
Marangoni AG, Barbut S, McGauley SE et al (2000) On the structure of particulate gels—the case of salt-induced cold gelation of heat-denatured whey protein isolate. Food Hydrocoll 14:61–74. https://doi.org/10.1016/S0268-005X(99)00046-6
Marino R, Giovando S, Gabriele D (2014) Effect of tannin addition on the rheological properties of starch-based adhesives. Appl Rheol 24:46138-(1:10). https://doi.org/10.3933/ApplRheol-24-46138
Meza BE, Verdini RA, Rubiolo AC (2009) Viscoelastic behaviour of heat-treated whey protein concentrate suspensions. Food Hydrocoll 23:661–666. https://doi.org/10.1016/J.FOODHYD.2008.03.015
Moelants KRN, Cardinaels R, Jolie RP, Verrijssen TAJ, Buggenhout S, Zumalacarregui LM, Loey AM, Moldenaers P, Hendrickx ME (2013) Relation between particle properties and rheological characteristics of carrot-derived suspensions. Food Bioprocess Technol 6:1127–1143. https://doi.org/10.1007/s11947-011-0718-0
Morison KR, Mackay FM (2001) Viscosity of lactose and whey protein solutions. Int J Food Prop 4:441–454. https://doi.org/10.1081/JFP-100108647
Muthukumar M (1989) Screening effect on viscoelasticity near the gel point. Macromolecules 22:4656–4658. https://doi.org/10.1021/ma00202a050
Navarro-Verdugo AL, Goycoolea FM, Romero-Meléndez G et al (2011) A modified Boltzmann sigmoidal model for the phase transition of smart gels. Soft Matter 7:5847. https://doi.org/10.1039/c1sm05252g
Nicolai T, Britten M, Schmitt C (2011) β-Lactoglobulin and WPI aggregates: formation, structure and applications. Food Hydrocoll 25:1945–1962. https://doi.org/10.1016/J.FOODHYD.2011.02.006
Nicolás P, Ferreira ML, Lassalle V (2019) A review of magnetic separation of whey proteins and potential application to whey proteins recovery, isolation and utilization. J Food Eng 246:7–15. https://doi.org/10.1016/J.JFOODENG.2018.10.021
Olsztyńska-Janus S, Pietruszka A, Kiełbowicz Z, Czarnecki MA (2018) ATR-IR study of skin components: lipids, proteins and water. Part I: temperature effect. Spectrochim Acta Part A Mol Biomol Spectrosc 188:37–49. https://doi.org/10.1016/J.SAA.2017.07.001
Ould Eleya MM, Ko S, Gunasekaran S (2004) Scaling and fractal analysis of viscoelastic properties of heat-induced protein gels. Food Hydrocoll 18:315–323. https://doi.org/10.1016/S0268-005X(03)00087-0
Pabst W, Gregorová E, Berthold C (2006) Particle shape and suspension rheology of short-fiber systems. J Eur Ceram Soc 26:149–160. https://doi.org/10.1016/J.JEURCERAMSOC.2004.10.016
Pelegrine DHG, Gasparetto CA (2005) Whey proteins solubility as function of temperature and pH. LWT Food Sci Technol 38:77–80. https://doi.org/10.1016/J.LWT.2004.03.013
Raikos V (2010) Effect of heat treatment on milk protein functionality at emulsion interfaces. A review. Food Hydrocoll 24:259–265. https://doi.org/10.1016/J.FOODHYD.2009.10.014
Rao MA (1999) Rheology of fluid and semisolid foods principles and applications. Aspen Publishers Inc, Gaithersburg
Rueb CJ, Zukoski CF (1997) Viscoelastic properties of colloidal gels. J Rheol 41:197–218. https://doi.org/10.1122/1.550812
Schröder A, Berton-Carabin C, Venema P, Cornacchia L (2017) Interfacial properties of whey protein and whey protein hydrolysates and their influence on O/W emulsion stability. Food Hydrocoll 73:129–140. https://doi.org/10.1016/J.FOODHYD.2017.06.001
Seta L, Baldino N, Gabriele D et al (2013) The influence of carrageenan on interfacial properties and short-term stability of milk whey proteins emulsions. Food Hydrocoll 32:373–382. https://doi.org/10.1016/j.foodhyd.2013.01.020
Seta L, Baldino N, Gabriele D et al (2014) Rheology and adsorption behaviour of β-casein and β-lactoglobulin mixed layers at the sunflower oil/water interface. Colloids Surfaces A Physicochem Eng Asp 441:669–677. https://doi.org/10.1016/j.colsurfa.2013.10.041
Shahbandeh M (2018) Value of the whey protein market worldwide from 2017 to 2023 (in billion U.S. dollars). https://www.statista.com/statistics/728005/global-whey-protein-market-size/. Accessed May 2019
Shih W-H, Shih WY, Kim S-I et al (1990) Scaling behavior of the elastic properties of colloidal gels. Phys Rev A 42:4772–4779. https://doi.org/10.1103/PhysRevA.42.4772
Smithers GW (2008) Whey and whey proteins—from ‘gutter-to-gold.’. Int Dairy J 18:695–704. https://doi.org/10.1016/J.IDAIRYJ.2008.03.008
Talari ACS, Martinez MAG, Movasaghi Z et al (2017) Advances in Fourier transform infrared (FTIR) spectroscopy of biological tissues. Appl Spectrosc Rev 52:456–506. https://doi.org/10.1080/05704928.2016.1230863
Tang D, Marangoni AG (2008) Modified fractal model and rheological properties of colloidal networks. J Colloid Interface Sci 318:202–209. https://doi.org/10.1016/j.jcis.2007.09.062
van der Werff JC, de Kruif CG (1989) Hard-sphere colloidal dispersions: the scaling of rheological properties with particle size, volume fraction, and shear rate. J Rheol 33:421–454. https://doi.org/10.1122/1.550062
Vardhanabhuti B, Foegeding EA (1999) Rheological properties and characterization of polymerized whey protein isolates. J Agric Food Chem 47:3649–3655. https://doi.org/10.1021/jf981376n
Wagoner TB, Foegeding EA (2017) Whey protein–pectin soluble complexes for beverage applications. Food Hydrocoll 63:130–138. https://doi.org/10.1016/J.FOODHYD.2016.08.027
Widjanarko SB, Nugroho A, Estiasih T (2011) Functional interaction components of protein isolates and glucomannan in food bars by FTIR and SEM studies. Afr J Food Sci 5:12–21
Zand-Rajabi H, Madadlou A (2016) Caffeine-loaded whey protein hydrogels reinforced with gellan and enriched with calcium chloride. Int Dairy J 56:38–44. https://doi.org/10.1016/J.IDAIRYJ.2015.12.011
Zhang Z, Arrighi V, Campbell L et al (2018) Properties of partially denatured whey protein products: viscoelastic properties. Food Hydrocoll 80:298–308. https://doi.org/10.1016/J.FOODHYD.2017.12.039
Funding
This work received financial support from Regione Calabria through the project POR CALABRIA FESR 2014/2020 Azione 1.2.2 “Recupero degli scarti di lavorazione nell’industria lattiero casearia per la produzione di cibi proteici” (Healthy Whey)—CUP J88C17000370006
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Lupi, F.R., Franco, G., Baldino, N. et al. The effect of operating conditions on the physicochemical characteristics of whey protein–based systems. Rheol Acta 59, 227–238 (2020). https://doi.org/10.1007/s00397-020-01197-6
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DOI: https://doi.org/10.1007/s00397-020-01197-6