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Modified screen-printed electrodes for the investigation of the interaction of non-electroactive quinazoline derivatives with DNA

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Abstract

Five morpholino-quinazoline derivatives have been investigated voltammetrically using a competition with the tris(o-phenanthroline) cobalt(III) redox marker for the accumulation at dsDNA modified screen-printed electrodes. An association of quinazolines with DNA was observed at the modified electrodes polarized by the negative potential of –0.4 V vs. Ag/AgCl. This was confirmed by a potentiometric stripping analysis based on the DNA guanine signal. Calibration curves for quinazolines within a concentration range of μmol/L were obtained with DP voltammetry using 5 × 10–7 mol/L Co(phen)3 3+ marker. The quinazolines exhibit no effect on the DNA complex with the fluorescent thiazole orange derivative TO-PRO-3. The role of the accumulation potential in the association interaction with DNA is discussed.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Slovak Technical University, SK-81237 Bratislava, Slovakia, e-mail: labuda@chtf.stuba.sk, , , , , , XX

    J. Labuda & M. Bučková

  2. Department of Microbiology, Biochemistry and Biology, Slovak Technical University, SK-81237 Bratislava, Slovakia, , , , , , XX

    S. Jantová

  3. FACH, SK-080 06 Prešov, Slovakia, , , , , , XX

    I. Štepánek

  4. Department of Pure and Applied Biochemistry, Lund University, S-22100 Lund, Sweden, , , , , , SE

    I. Surugiu & B. Danielsson

  5. Department of Public Health, Environmental and Analytical Chemistry, University of Firenze, I-50121 Firenze, Italy, , , , , , IT

    M. Mascini

Authors
  1. J. Labuda
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  2. M. Bučková
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  3. S. Jantová
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  4. I. Štepánek
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  5. I. Surugiu
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  6. B. Danielsson
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  7. M. Mascini
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Additional information

Received: 29 December 1999 / Revised: 1 March 2000 / Accepted: 7 March 2000

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Labuda, J., Bučková, M., Jantová, S. et al. Modified screen-printed electrodes for the investigation of the interaction of non-electroactive quinazoline derivatives with DNA. Fresenius J Anal Chem 367, 364–368 (2000). https://doi.org/10.1007/s002160000388

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  • DOI: https://doi.org/10.1007/s002160000388

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