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Amperometric biogenic amine biosensors based on Prussian blue, indium tin oxide nanoparticles and diamine oxidase– or monoamine oxidase–modified electrodes

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Abstract

Biogenic amine biosensors, based on screen-printed carbon electrodes (SPCE) modified with Prussian blue (PB) and indium tin oxide nanoparticles (ITONP), are reported. PB/ITONP-modified SPCE was further modified with diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes to construct the biosensors. The morphology of the modified electrodes was studied by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to enlighten the electrochemical properties of the modified electrodes at each step of biosensor fabrication. Electrode surface composition and experimental conditions were optimized and analytical performance characteristics of the biosensors were studied. Several biogenic amines were tested and both biosensors responded to histamine, putrescine and cadaverine. DAO/ITONP/PB/SPCE biosensor exhibited the highest response to histamine 6.0 × 10−6–6.9 × 10−4 M with a sensitivity of 1.84 μA mM−1. On the other hand, the highest sensitivity was obtained for cadaverine with the MAO/ITONP/PB/SPCE biosensor. The analytical utility of the presented biosensors were illustrated by the determination of cadaverine and histamine in cheese sample.

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Funding

This work was financially supported by The Scientific and Technical Research Council of Turkey (TUBITAK Project No.: 116Z159).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Ankara University, 06100, Ankara, Turkey

    Ceren Kaçar, Berna Dalkiran, Emine Kübra İnal & Esma Kiliç

  2. Department of Chemistry, Polatlı Faculty of Science and Arts, Ankara Hacı Bayram Veli University, 06900, Ankara, Turkey

    Pınar Esra Erden

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  1. Ceren Kaçar
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Kaçar, C., Erden, P.E., Dalkiran, B. et al. Amperometric biogenic amine biosensors based on Prussian blue, indium tin oxide nanoparticles and diamine oxidase– or monoamine oxidase–modified electrodes. Anal Bioanal Chem 412, 1933–1946 (2020). https://doi.org/10.1007/s00216-020-02448-4

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