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New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion

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Abstract

A new near-infrared-based photothermal immunosensing strategy was developed for the sensitive and feasible detection of human chorionic gonadotropin (HCG) by use of a Prussian blue nanoparticle-based photothermal conversion system. Prussian blue nanospheres synthesized by the one-pot method were used for the labeling of anti-HCG detection antibody. A sandwich-type immunoreaction was initially conducted on a monoclonal anti-HCG antibody-coated microplate with a nanoparticle-labeled signal antibody. Accompanying formation of the sandwiched immunocomplex, Prussian blue nanospheres caused photothermal conversion under 980-nm laser irradiation, thereby resulting in an increase of the temperature of the detection system measured by a portable digital thermometer. The properties and factors influencing the analytical performance of the photothermal immunoassay were studied in detail. Under the optimal conditions, the Prussian blue nanoparticle-based photothermal immunoassay exhibited good temperature responses relative to target HCG concentrations within the dynamic range of 0.01–100 ng mL-1 at a low detection limit of 5.8 pg mL-1. This system also displayed good anti-interference behavior with regard to other cancer biomarkers, good reproducibility, and relatively long storage stability. The method accuracy was evaluated for analysis of human serum specimens, giving results that matched well with those obtained with a commercial HCG enzyme-linked immunosorbent assay kit. Importantly, this protocol is promising for advanced development of photothermal immunoassays.

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Funding

We sincerely acknowledge the financial support of the National Natural Science Foundation of China (81772287 and 81371902), the Joint Project of Major Diseases in Xiamen City of China (3502Z20179044), and the Natural Science Foundation of Fujian Province, China (2016J01643).

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Author notes

  1. Guolin Hong and Dongdong Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, 361005, Fujian, China

    Guolin Hong, Dongdong Zhang, Yinghao He, Yuanyuan Yang & Ping Chen

  2. Department of Clinical Medicine, Fujian Medical University, Fuzhou, 350108, Fujian, China

    Guolin Hong, Huijing Yang & Zhiyang Zhou

  3. Department of Laboratory Medicine, Fuzhou Second Hospital Affiliated to Xiamen University, Fuzhou, 350007, Fujian, China

    Yinhuan Liu

  4. Department of Cardiology, The Affiliated Cardiovascular Hospital of Xiamen University, Medical College of Xiamen University, Xiamen, 361004, Fujian, China

    Yan Wang

Authors
  1. Guolin Hong
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  2. Dongdong Zhang
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  3. Yinghao He
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  4. Yuanyuan Yang
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  6. Huijing Yang
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  8. Yinhuan Liu
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  9. Yan Wang
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Corresponding authors

Correspondence to Yinhuan Liu or Yan Wang.

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The authors declare that they have no competing interests.

All procedures performed in studies involving human participants were approved by the First Affiliated Hospital of Xiamen University and Fujian Medical University and in accordance with the ethical standards of the First Affiliated Hospital of Xiamen University and Fujian Medical University ethics committees and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Hong, G., Zhang, D., He, Y. et al. New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion. Anal Bioanal Chem 411, 6837–6845 (2019). https://doi.org/10.1007/s00216-019-02049-w

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  • DOI: https://doi.org/10.1007/s00216-019-02049-w

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