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Highly sensitive electrochemical immunoassay for human IgG using double-encoded magnetic redox-active nanoparticles

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Abstract

A new sandwich-type electrochemical immunoassay was developed for the detection of human IgG using doubly-encoded and magnetic redox-active nanoparticles as recognition elements on the surface of a glassy carbon electrode modified with anti-IgG on nanogold particles. The recognition elements were synthesized by coating magnetic Fe3O4 nanoparticles with Prussian blue nanoparticles and then covered with peroxidase-labeled anti-IgG antibodies (POx-anti-IgG) on Prussian blue nanoparticles. The immunoelectrode displays very good electrochemical properties towards detection of IgG via using double-encoded magnetic redox-active nanoparticles as trace and hydrogen peroxide as enzyme substrate. Its limit of detection (10 pmol·L−1) is 10-fold better than that of using plain POx-anti-IgG secondary antibodies. The method was applied to the detection of IgG in serum samples, and an excellent correspondence with the reference values was found.

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Acknowledgement

The authors appreciate the support of the High-Qualified Talent Foundation of Fuzhou University (0460-022275), the National Basic Research Program of China (2010CB732403), the National Natural Science Foundation of China (20877019, 20735002), and NTU-MOE Academic Research Funds (RG65/08).

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Authors and Affiliations

  1. Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education), Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Dianping Tang, Juan Tang, Biling Su, Huafeng Chen & Guonan Chen

  2. Clinical Laboratory and Medical Diagnostics Laboratory, Fujian Provincial Hospital, Fujian, 350001, People’s Republic of China

    Jianxin Huang

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  1. Dianping Tang
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  2. Juan Tang
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  3. Biling Su
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  4. Huafeng Chen
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  5. Jianxin Huang
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  6. Guonan Chen
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Correspondence to Dianping Tang.

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Tang, D., Tang, J., Su, B. et al. Highly sensitive electrochemical immunoassay for human IgG using double-encoded magnetic redox-active nanoparticles. Microchim Acta 171, 457–464 (2010). https://doi.org/10.1007/s00604-010-0442-x

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  • DOI: https://doi.org/10.1007/s00604-010-0442-x

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