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Nanoparticles for nucleic-acid-based biosensing: opportunities, challenges, and prospects

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Abstract

Electrochemical nucleic-acid-based biosensing strategies involving the use of nanoparticles as electrode modifiers and as advanced labels are attractive options for the determination of substances that are relevant clinically, from an environmental perspective, and to food analysis, as these strategies are able to overcome some of the well-known limitations of conventional methodologies for routine applications. In this article, we provide a selective overview of current strategies for nucleic acid electrochemical biosensing based on nanoparticles, in order to demonstrate the relevance and potential of these strategies to readers familiar with this field and to non-experts. The benefits provided by the use of nanoparticles, including enhanced analytical performance of the resulting electrochemical biosensors, as well as the main challenges to be solved and potential future advances in this field are discussed.

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Acknowledgements

The financial support of the CTQ2015-70023-R and CTQ2015-64402-C2-1-R (Spanish Ministerio de Economía y Competitividad Research Projects) and S2013/MT3029 (NANOAVANSENS Program from the Comunidad de Madrid) are gratefully acknowledged.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, 28040, Madrid, Spain

    Susana Campuzano, Paloma Yáñez-Sedeño & José M. Pingarrón

  2. IMDEA Nanoscience, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain

    José M. Pingarrón

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  1. Susana Campuzano
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  2. Paloma Yáñez-Sedeño
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  3. José M. Pingarrón
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Correspondence to Susana Campuzano or José M. Pingarrón.

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Published in the topical collection Nanoparticles for Bioanalysis with guest editors María Carmen Blanco-López and Montserrat Rivas.

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Campuzano, S., Yáñez-Sedeño, P. & Pingarrón, J.M. Nanoparticles for nucleic-acid-based biosensing: opportunities, challenges, and prospects. Anal Bioanal Chem 411, 1791–1806 (2019). https://doi.org/10.1007/s00216-018-1273-6

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