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Catalytic chemiluminescent detection of cholesterol in serum with Cu2−x Se semiconductor nanoparticles

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Abstract

It is very important to be able to accurately and rapidly measure the cholesterol level in the human body, as cholesterol is associated with various diseases, such as Alzheimer’s disease. In this work, a novel method of detecting cholesterol using chemiluminescence (CL) based on a newly prepared semiconductor catalyst, Cu2−x Se nanoparticles (Cu2−x Se NPs), was developed. It was found that the Cu2−x Se NPs strongly enhanced the CL signal by producing a large number of reactive oxygen species (ROS) in the luminol–Cu2−x Se NPs system. Based on the UV-vis-NIR absorption spectra, zeta potential, CL spectrum, and an investigation of the ROS, a possible mechanism for the CL was proposed. This CL-based method was successfully applied to determine cholesterol. It was found that the enhanced CL was proportional to the concentration of cholesterol over the range of 82 nM to 1.96 μM with a detection limit of 0.062 nM, and that added cholesterol was successfully detected in human serum with a mean recovery of 97 %.

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Acknowledgments

The present work was financially supported by the National Natural Science Foundation of China (NSFC, no. 21375109). The authors appreciate the kind help of Sireesh Babu Maddinedi in relation to manuscript editing.

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Authors and Affiliations

  1. Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Fang Fang Zhang, Hong Wang & Cheng Zhi Huang

  2. College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China

    Hong Yan Zou, Jing Lan & Cheng Zhi Huang

Authors
  1. Fang Fang Zhang
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  2. Hong Yan Zou
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  3. Jing Lan
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  4. Hong Wang
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Corresponding author

Correspondence to Cheng Zhi Huang.

Ethics declarations

The use of human serum was approved by the University of Southwest’s Institutional Review Board. A signed individual written informed consent agreement was obtained from the participants before beginning the work on this study, and the research was not performed outside our country of residence.

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The authors declare no competing financial interest.

Additional information

Published in the topical collection Highlights of Analytical Chemical Luminescence with guest editors Aldo Roda, Hua Cui, and Chao Lu.

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Zhang, F.F., Zou, H.Y., Lan, J. et al. Catalytic chemiluminescent detection of cholesterol in serum with Cu2−x Se semiconductor nanoparticles. Anal Bioanal Chem 408, 8771–8778 (2016). https://doi.org/10.1007/s00216-016-9342-1

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  • DOI: https://doi.org/10.1007/s00216-016-9342-1

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