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Iron oxide/tantalum oxide core–shell magnetic nanoparticle-based microwave-assisted extraction for phosphopeptide enrichment from complex samples for MALDI MS analysis

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Abstract

A new type of metal-oxide-coated magnetic nanoparticles (NPs)—tantalum-oxide-coated magnetic iron oxide (Fe3O4@Ta2O5) NPs—which are used as affinity probes for selectively trapping phosphopeptides from complex samples, is demonstrated in this study. In this approach, phosphopeptide enrichment was achieved by incubating the NPs with sample solutions under microwave heating within 1 min. The NP–target species conjugates were readily isolated from samples by magnetic separation followed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis. When using human serum as the sample, phosphorylated fibrinopeptide-A-derived ions are the only ions observed in the MALDI mass spectra after enrichment by the Fe3O4@Ta2O5 NPs. Furthermore, only phosphopeptides appear in the MALDI mass spectra after using the affinity probes to selectively trap target species from the tryptic digest of a cell lysate and milk sample. The results demonstrated that the Fe3O4@Ta2O5 NPs have the capability of selectively trapping phosphorylated peptides from complex samples. The detection limit of this approach for a phosphopeptide (FQpSEEQQQTEDELQDK) was ~10 fmol.

For the first time, tantalum oxide-coated magnetic iron oxide (Fe3O4@Ta2O5) NPs were demonstrated as suitable affinity-probes for selectively trapping phosphopeptides from complex samples. To shorten the analysis time, phosphopeptide enrichment was achieved by incubating the NPs with sample solutions under microwave-heating within 1 min. MALDI MS was employed for characterization of the species trapped by the NPs.

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Acknowledgment

We thank the National Science Council of Taiwan and MOE-ATU (96W821G021 and 96W801H109) for supporting this research financially.

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Authors and Affiliations

  1. Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan

    Hong-Yi Lin, Wei-Yu Chen & Yu-Chie Chen

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  1. Hong-Yi Lin
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  2. Wei-Yu Chen
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  3. Yu-Chie Chen
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Correspondence to Yu-Chie Chen.

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Lin, HY., Chen, WY. & Chen, YC. Iron oxide/tantalum oxide core–shell magnetic nanoparticle-based microwave-assisted extraction for phosphopeptide enrichment from complex samples for MALDI MS analysis. Anal Bioanal Chem 394, 2129–2136 (2009). https://doi.org/10.1007/s00216-009-2890-x

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  • DOI: https://doi.org/10.1007/s00216-009-2890-x

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