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Enzyme-linked immunosorbent assay in analysis of deoxynivalenol: investigation of the impact of sample matrix on results accuracy

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Abstract

Enzyme-linked immunosorbent assay (ELISA) represents a bioanalytical strategy frequently used for rapid screening of mycotoxin deoxynivalenol (DON) in cereals and derived products. Due to a considerable affinity of some anti-DON antibodies to structurally similar DON metabolites, such as DON-3-glucoside (DON-3-Glc) and 3-acetyl-DON (3-ADON), a significant overestimation of DON concentrations may occur. A validation study of six commercial DON-dedicated ELISA kits, namely Ridascreen DON, Ridascreen FAST, DON, DON EIA, AgraQuant DON Assay, Veratox 5/5, and Veratox HS was carried out on wheat, barley, and malt matrices. Performance characteristics of all tested ELISAs were determined using aqueous solutions of DON, DON-3-Glc, and 3-ADON analytical standards, further with extracts of artificially spiked blank cereals, and finally with matrix-matched standards of all three compounds. In the final phase, the accuracy of data was assessed through a comparison of DON concentrations determined by particular ELISAs and reference ultra-high-performance liquid chromatography–tandem mass spectrometry method. For this purpose, both quality control materials and a comprehensive set of naturally and artificially contaminated samples of wheat, barley, and malt were analyzed. High cross-reactivities were proved for both DON-3-Glc and 3-ADON in the majority of examined assays, and moreover, a considerable contribution of some matrix components to overestimation of DON results was confirmed.

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References

  1. Richard JL (2007) Some major mycotoxins and their mycotoxicoses—an overview. Int J Food Microbiol 119:3–10

    Article  CAS  Google Scholar 

  2. Poppenberger B, Berthiller F, Lucyshyn D, Sieberer T, Schuhmacher R, Krska R, Kuchler K, Glossl J, Luschnig C, Adam G (2003) Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana. J Biol Chem 278:47905–47914

    Article  CAS  Google Scholar 

  3. Kostelanska M, Hajslova J, Zachariasova M, Malachova A, Kalachova K, Poustka J, Fiala J, Scott PM, Berthiller F, Krska R (2009) Occurrence of deoxynivalenol and its major conjugate, deoxynivalenol-3-glucoside, in beer and some brewing intermediates. J Agric Food Chem 57:3187–3194

    Article  CAS  Google Scholar 

  4. Berthiller F, Dall’Asta C, Corradini R, Marchelli R, Sulyok M, Krska R, Adam G, Schuhmacher R (2009) Occurrence of deoxynivalenol and its 3-β-d-glucoside in wheat and maize. Food Add Contam 26:507–511

    Article  CAS  Google Scholar 

  5. Malachova A, Dzuman Z, Veprikova Z, Vaclavikova M, Zachariasova M, Hajslova J (2011) Deoxynivalenol, deoxynivalenol-3-glucoside and enniatins: the major mycotoxins found in cereal-based products in the Czech market. J Agric Food Chem 59:12990–12997

    Article  CAS  Google Scholar 

  6. Berthiller F, Crews C, Dall’Asta C, De Saeger S, Haesaert G, Karlovsky P, Oswald IP, Seefelder W, Speijers G, Stroka J (2013) Masked mycotoxins: a review. Mol Nutr Food Res 57:165–186

    Article  CAS  Google Scholar 

  7. Vishwanath V, Sulyok M, Labuda R, Bicker W, Krska R (2009) Simultaneous determination of 186 fungal and bacterial metabolites in indoor matrices by liquid chromatography/tandem mass spectrometry. Anal Bioanal Chem 395:1355–1372

    Article  CAS  Google Scholar 

  8. Lacina O, Zachariasova M, Urbanova J, Vaclavikova M, Cajka T, Hajslova J (2012) Critical assessment of extraction methods for the simultaneous determination of pesticide residues and mycotoxins in fruits, cereals, spices and oil seeds employing ultra-high performance liquid chromatography–tandem mass spectrometry. J Chromatogr A 1262:8–18

    Article  CAS  Google Scholar 

  9. Commission Regulation (EC) (2006) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union L 364:5–24

    Google Scholar 

  10. Commission Regulation (EC) (2007) No 1126/2007 of 28 September 2007 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Off J Eur Union L 255:14–17

    Google Scholar 

  11. Seventy-second report of the Joint FAO/WHO Expert Committee on Food Additives, WHO Technical Report Series 959, Evaluation of certain contaminants in food, report TRS 906-JECFA 56/35. http://whqlibdoc.who.int/trs/WHO_TRS_959_eng.pdf. Accessed 2 Jul 2013

  12. Dall’Erta A, Cirlini M, Dall’Asta M, Del Rio M, Galaverna G, Dall’Asta C (2013) Masked mycotoxins are efficiently hydrolyzed by human colonic microbiota releasing their aglycones. Chem Res Toxicol 26:305–312

    Article  Google Scholar 

  13. Schneider E, Curtui V, Seidler C, Dietrich R, Usleber E, Martlbauer E (2004) Rapid methods for deoxynivalenol and other trichothecenes. Tox Lett 153:113–121

    Article  CAS  Google Scholar 

  14. Zheng MZ, Richard JL, Binder J (2006) A review of rapid methods for the analysis of mycotoxins. Mycopathologia 161:261–273

    Article  CAS  Google Scholar 

  15. Tangni EK, Motte JC, Callebaut A, Pussemier L (2010) Cross-reactivity of antibodies in some commercial deoxynivalenol test kits against some fusariotoxins. J Agric Food Chem 58:12625–12633

    Article  CAS  Google Scholar 

  16. Pascale MN (2009) Detection methods for mycotoxins in cereal grains and cereal products. Proc Natl Acad Sci U S A 117:15–25

    CAS  Google Scholar 

  17. Zhang A, Ma Y, Feng L, Wang Y, He C, Wang X, Zhang H (2011) Development of a sensitive competitive indirect ELISA method for determination of ochratoxin A levels in cereal originating from Nanjing, China. Food Control 22:1723–1728

    Article  CAS  Google Scholar 

  18. Xu Y, Huang ZB, He QH, Deng SZ, Li LS, Li YP (2010) Development of an immunochromatographic strip test for the rapid detection of deoxynivalenol in wheat and maize. Food Chem 119:834–839

    Article  CAS  Google Scholar 

  19. Folloni S, Bellocchi G, Kagkli DM, Pastor-Benito S, Aguilera M, Mazzeo A, Querci M, Van der Eede G, Ermolli M (2011) Development of an ELISA reverse-based assay to assess the presence of mycotoxins in cereal flour. Food Anal Methods 4:221–227

    Article  Google Scholar 

  20. Bolduan C, Montes JM, Dhillon BS, Mirdita V, Melchinger AE (2009) Determination of mycotoxin by ELISA and near-infrared spectroscopy in Fusarium-inoculated maize. Cereal Res Comm 37:521–529

    Article  CAS  Google Scholar 

  21. Zachariasova M, Hajslova J, Kostelanska M, Poustka J, Krplova A, Cuhra P, Hochel I (2008) Deoxynivalenol and its conjugates in beer: a critical assessment of data obtained by enzyme-linked immunosorbent assay and liquid chromatography coupled to tandem mass spectrometry. Anal Chim Acta 625:77–86

    Article  CAS  Google Scholar 

  22. Ruprich J, Ostry V (2008) Immunochemical methods in health risk assessment: cross-reactivity of antibodies against mycotoxin deoxynivalenol with deoxynivalenol-3-glucoside. Cent Eur J Publ Health 16:34–37

    CAS  Google Scholar 

  23. Tran ST, Smith TK, Girgis GN (2011) A survey of free and conjugated deoxynivalenol in the 2008 corn crop in Ontario, Canada. J Sci Food Agric 92:37–41

    Article  Google Scholar 

Download references

Acknowledgments

This study was financially supported by national projects provided by Ministry of Education, Youth and Sports of the Czech Republic NPV II 2B08049 and partly by Ministry of Agricultural of the Czech Republic within the project QI111B154.

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Authors and Affiliations

  1. ICT Prague, Technicka 3, 166-28, Prague, Czech Republic

    Zbynek Dzuman, Marta Vaclavikova, Zdenka Veprikova, Marie Fenclova, Milena Zachariasova & Jana Hajslova

  2. Agrotest fyto Ltd, Havlickova 121, 767-01, Kromeriz, Czech Republic

    Ivana Polisenska

Authors
  1. Zbynek Dzuman
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  2. Marta Vaclavikova
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  3. Ivana Polisenska
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  4. Zdenka Veprikova
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  5. Marie Fenclova
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  6. Milena Zachariasova
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  7. Jana Hajslova
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Correspondence to Jana Hajslova.

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Dzuman, Z., Vaclavikova, M., Polisenska, I. et al. Enzyme-linked immunosorbent assay in analysis of deoxynivalenol: investigation of the impact of sample matrix on results accuracy. Anal Bioanal Chem 406, 505–514 (2014). https://doi.org/10.1007/s00216-013-7463-3

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  • DOI: https://doi.org/10.1007/s00216-013-7463-3

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