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A Review of Rapid Methods for the Analysis of Mycotoxins

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Abstract

An overview is presented of the analysis of mycotoxins by rapid methods such as: enzyme linked immuno-sorbent assay (ELISA); flow through membrane based immunoassay; immunochromatographic assay; fluorometric assay with immunoaffinity clean-up column or with a solid phase extraction clean-up column; and fluorescence polarization method. These methods are currently commercially available and are reliable, rapid methods. This review focuses on the basic principle of each rapid method as well as advantages and limitations of each method. Additionally, we address other emerging technologies of potential application in the analysis of mycotoxins.

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References

  1. Council for Agricultural Science and Technology (CAST). Mycotoxins: Risks in Plant, Animal and Human Systems. Task Force Report, No. 139, CAST, Ames, Iowa, 2003.

  2. HP Egmond ParticleVan MA Jonker (2004) Worldwide Regulations for Mycotoxins in Food and Feed in 2003 Food and Agriculture Organization of the United Nations Rome

    Google Scholar 

  3. Commission Regulation (EC) No 466/2001 of 8 March 2001 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Communities 2001; L77: 1–13.

    Google Scholar 

  4. Commission Regulation (EC) No 472/2002 of 12 March 2002 amending Regulation (EC) No 466/2001 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Communities 2002; L75: 18–20.

    Google Scholar 

  5. Directive 2002/32/EC of the European Parliament and of the Council of 7 May 2002 on undesirable substances in animal feed. Official Journal of the European Communities 2002; L140: 10–21.

    Google Scholar 

  6. TB Whitaker JW Dickens (1979) ArticleTitleVariability Associated with Testing Corn for Aflatoxin J Assoc Off Anal Chem 56 789–794 Occurrence Handle1:CAS:528:DyaE1MXlvFWnt78%3D

    CAS  Google Scholar 

  7. FP Scanlan (2005) Why rapid testing? A Amerongen Particlevan D Barug M Lauwaars (Eds) Rapid Methods for Biological, Chemical Contaminants in Food and Feed Wageningen Academic Publishers The Netherlands 19–29

    Google Scholar 

  8. CM Maragos (2004) ArticleTitleEmerging Technologies for Mycotoxin Detection J Toxicol Toxin Rev 23 317–344 Occurrence Handle10.1081/TXR-200027859 Occurrence Handle1:CAS:528:DC%2BD2cXnt1GksL0%3D

    Article  CAS  Google Scholar 

  9. Maragos CM. Recent advances in analytical methods for mycotoxins. New Horizon of Mycotoxicology for Assuring Food Safety (Proceedings of ISMYCO Kagawa’03), 2003: 215–223.

  10. USDA/GIPSA. Design criteria and test performance specifications for quantitative aflatoxin test kits. US Department of Agriculture, Federal Grain Inspection Service Quality Assurance and Research Division. 1998.

  11. FS Chu (1996) Recent studies on immunoassays for mycotoxin RC Beier LH Stanker (Eds) Immunoassays for Residue Analysis American Chemical Society Washington, DC 294–313

    Google Scholar 

  12. MW Trucksess (2001) Rapid analysis (thin layer chromatographic and immunochemical methods) for mycotoxins in foods and feeds) WJ Koe Particlede RA Samson HP van Egmond J Gilbert M Sabino (Eds) Mycotoxins and Phycotoxins in Perspective at the Turn of the Millennium W.J. de Koe, Wageningen The Netherlands 29–40

    Google Scholar 

  13. MW Trucksess DE Koeltzow (1995) Evaluation and application of immunochemical methods for mycotoxins in food JO Nelson AE Karu RB Wong (Eds) Immunoanalysis of Agrochemicals in Emerging Technologies American Chemical Society Washington, DC 326–334

    Google Scholar 

  14. J Gilbert E Anklam (2002) ArticleTitleValidation of analytical methods for determining mycotoxins in foodstuffs Trends Anal Chem 21 IssueID6–7 468–486 Occurrence Handle10.1016/S0165-9936(02)00604-0 Occurrence Handle1:CAS:528:DC%2BD38Xltlamtrs%3D

    Article  CAS  Google Scholar 

  15. Z Zheng CW Humphrey RS King JL Richard (2005) ArticleTitleValidation of an ELISA test kit for the detection of total Aflatoxins in grain and grain products Mycopathologia 159 IssueID2 255–263 Occurrence Handle10.1007/s11046-004-8666-0 Occurrence Handle1:CAS:528:DC%2BD2MXitlOjurg%3D Occurrence Handle15770452

    Article  CAS  PubMed  Google Scholar 

  16. MW Trucksess ME Stack S Nesheim DL Park AE Pohland (1989) ArticleTitleEnzyme-linked immunosorbent assay of Aflatoxins B1, B2, and G1 in corn, cottonseed, peanuts, peanut butter, and poultry feed: collaborative Study J Assoc Off Anal Chem 72 IssueID6 957–962 Occurrence Handle1:CAS:528:DyaK3cXjsVCqtA%3D%3D Occurrence Handle2512279

    CAS  PubMed  Google Scholar 

  17. MW Trucksess ME Stack (1994) ArticleTitleEnzyme-linked immunosorbent assay of total aflatoxins B1, B2 and G1 in corn: follow-up collaborative study J AOAC Int 77 IssueID3 655–658 Occurrence Handle1:CAS:528:DyaK2cXktlenuro%3D Occurrence Handle8012215

    CAS  PubMed  Google Scholar 

  18. C Paepens S Saeger ParticleDe L Sibanda I Barna-Vetro I Leglise F Hove ParticleVan C Peteghem ParticleVan (2004) ArticleTitleA flow-through enzyme immunoassay for the screening of fumonisins in maize Analytica Chimica Acta 523 229–235 Occurrence Handle10.1016/j.aca.2004.07.044 Occurrence Handle1:CAS:528:DC%2BD2cXns12itro%3D

    Article  CAS  Google Scholar 

  19. Z Zheng ST Ku WS Ng J Binder (2005) A New AgraStripTM Total Aflatoxin Lateral Flow Test Kit. Poster Presentation in Gordon Research Conferences in Mycotoxins & Phycotoxins Colby College Waterville, ME, USA 19–24

    Google Scholar 

  20. PM Scott MW Trucksess (1997) ArticleTitleApplication of immunoaffinity columns to mycotoxin analysis J AOAC Int 80 941–949 Occurrence Handle1:CAS:528:DyaK2sXmtlymsbs%3D Occurrence Handle9325571

    CAS  PubMed  Google Scholar 

  21. MW Trucksess ME Stack S Nesheim SW Page RH Albert TJ Hansen KF Donahue (1991) ArticleTitleImmunoaffinity column coupled with solution fluorometry or LC post-column derivatization for aflatoxins in corn, peanuts, and peanut butter: collaborative study J AOAC Int 74 81–88 Occurrence Handle1:CAS:528:DyaK3MXht1Sgurw%3D

    CAS  Google Scholar 

  22. BR Malone CW Humphrey TR Romer JL Richard (2000) ArticleTitleDetermination of aflatoxins in grains and raw peanuts by a rapid procedure with fluorometric analysis J AOAC Int 83 95–98 Occurrence Handle1:CAS:528:DC%2BD3cXpvFGltw%3D%3D Occurrence Handle10693009

    CAS  PubMed  Google Scholar 

  23. BR Malone CW Humphrey TR Romer JL Richard (1998) ArticleTitleOne-step solid-phase extraction cleanup and fluorometric analysis of deoxynivalenol in grains J AOAC Int 81 IssueID2 448–452 Occurrence Handle1:CAS:528:DyaK1cXitleqsL0%3D Occurrence Handle9549079

    CAS  PubMed  Google Scholar 

  24. CM Maragos ME Jolley RD Plattner MS Nasir (2001) ArticleTitleFluorescence polarization as a means for determination of fumonisins in maize J Agric Food Chem 49 596–602 Occurrence Handle10.1021/jf0010782 Occurrence Handle1:CAS:528:DC%2BD3MXhsValtw%3D%3D Occurrence Handle11261998

    Article  CAS  PubMed  Google Scholar 

  25. CM Maragos E Kim (2004) ArticleTitleDetection of zearalenone and related metabolites by fluorescence polarization immunoassay J Food Protect 67 IssueID5 1039–1043 Occurrence Handle1:CAS:528:DC%2BD2cXksleqt7g%3D

    CAS  Google Scholar 

  26. CM Maragos RD Plattner (2002) ArticleTitleRapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat J Agric Food Chem 50 1827–1832 Occurrence Handle10.1021/jf011487d Occurrence Handle1:CAS:528:DC%2BD38XhsVGjsbk%3D Occurrence Handle11902919

    Article  CAS  PubMed  Google Scholar 

  27. U Jönson L Fägerstam B Ivarsson B Johnsson R Karlsson K Lundh S Löfas B Persson H Roos I Ronnberg (1991) ArticleTitleReal-time biospecific interaction analysis using surface resonance and a sensor chip technology BioTechniques 11 620–627

    Google Scholar 

  28. SJ Daly GJ Keating PP Dillon BM Manning R O’Kennedy HA Lee MRA Morgon (2000) ArticleTitleDevelopment of surface plasmon resonance-based immunoassay for aflatoxin B1 J Agric Food Chem 48 5097–5104 Occurrence Handle10.1021/jf9911693 Occurrence Handle1:CAS:528:DC%2BD3cXmvFartrk%3D Occurrence Handle11087443

    Article  CAS  PubMed  Google Scholar 

  29. W Mullett EPC Lai JM Yeung (1998) ArticleTitleImmunoassay of fumonisin by a surface plasmon resonance biosensor Anal Biochem 258 161–167 Occurrence Handle10.1006/abio.1998.2616 Occurrence Handle1:CAS:528:DyaK1cXjtFensL4%3D Occurrence Handle9570825

    Article  CAS  PubMed  Google Scholar 

  30. AJ Tudos ER Lucas-van Bos Particleden EC Stigter (2003) ArticleTitleRapid surface plasmon resonance-based inhibition assay of deoxynivalenol J Agric Food Chem 51 5843–5848 Occurrence Handle10.1021/jf030244d Occurrence Handle13129282

    Article  PubMed  Google Scholar 

  31. B Gaag ParticleVan der S Spath H Dietrich E Stigter G Boonzaaijer T Osenbruggen Particlevan K Koopal (2003) ArticleTitleBiosensors and multiple mycotoxin analysis Food Control 14 251–254 Occurrence Handle10.1016/S0956-7135(03)00008-2

    Article  Google Scholar 

  32. VS Thompson CM Maragos (1996) ArticleTitleFibre-optic immunosensor for the detection of fumonisin B1 J Agric Food Chem 44 1041–1046 Occurrence Handle10.1021/jf950661t Occurrence Handle1:CAS:528:DyaK28XhvVKns70%3D

    Article  CAS  Google Scholar 

  33. M Ulbricht H Matuschewski A Oechel HG Hicke (1996) ArticleTitlePhoto-induced graft polymerization surface modifications for the preparation of hydrophilic and low-protein-adsorbing ultrafiltration membrane J Membr Sci 115 31–47 Occurrence Handle10.1016/0376-7388(95)00264-2 Occurrence Handle1:CAS:528:DyaK28XjtlSru7c%3D

    Article  CAS  Google Scholar 

  34. NM Maier G Buttinger S Welhartizki E Gavioli W Lindner (2004) ArticleTitleMolecularly imprinted polymer-assisted sample clean-up of ochratoxin A from red wine: merits and limitations J Chromatogr B 804 103–111 Occurrence Handle10.1016/j.jchromb.2004.01.014 Occurrence Handle1:CAS:528:DC%2BD2cXjtVOjtrs%3D

    Article  CAS  Google Scholar 

  35. JCC Yu EPC Lai (2005) ArticleTitleInteraction of ochratoxin A with molecularly imprinted polypyrrole film on surface plasmon resonance sensor React Funct Polym 63 171–176 Occurrence Handle10.1016/j.reactfunctpolym.2005.03.001 Occurrence Handle1:CAS:528:DC%2BD2MXks1WksL0%3D

    Article  CAS  Google Scholar 

  36. DR Call MK Borucki FJ Loge (2003) ArticleTitleDetection of bacterial pathogens in environmental samples using DNA microarrays J Microbiol Methods 53 235–243 Occurrence Handle10.1016/S0167-7012(03)00027-7 Occurrence Handle1:CAS:528:DC%2BD3sXitlWru7c%3D Occurrence Handle12654494

    Article  CAS  PubMed  Google Scholar 

  37. M Nicolaisen AF Justesen U Thrane P Skouboe K Holmstrom (2005) ArticleTitleAn oligonucleotide microarray for the identification and differentiation of trichothecene producing and non-producing Fusarium species occurring on cereal grain J Microbiol Methods 62 57–69 Occurrence Handle10.1016/j.mimet.2005.01.009 Occurrence Handle1:CAS:528:DC%2BD2MXjtFyku7k%3D Occurrence Handle15823394

    Article  CAS  PubMed  Google Scholar 

  38. DA Vignali (2000) ArticleTitleMultiplexed practile-based flow cytometric assays J Immunol Methods 243 243–255 Occurrence Handle10.1016/S0022-1759(00)00238-6 Occurrence Handle1:CAS:528:DC%2BD3cXmtlGksr8%3D Occurrence Handle10986418

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Romer Labs Singapore Pte Ltd, 3791 Jalan Bukit Merah #08-08, eCentre@redhill building, Singapore, 159471, Singapore

    Michael Z. Zheng & Johann Binder

  2. Romer Labs, Inc., 1301 Stylemaster Dr., MO, 63084, USA

    John L. Richard

Authors
  1. Michael Z. Zheng
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  2. John L. Richard
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  3. Johann Binder
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Correspondence to Michael Z. Zheng.

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Zheng, M.Z., Richard, J.L. & Binder, J. A Review of Rapid Methods for the Analysis of Mycotoxins. Mycopathologia 161, 261–273 (2006). https://doi.org/10.1007/s11046-006-0215-6

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  • DOI: https://doi.org/10.1007/s11046-006-0215-6

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