Skip to main content
SpringerLink
Log in

An innovative, easily fabricated, silver nanoparticle-based titanium implant coating: development and analytical characterization

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Microbial colonization and biofilm formation on implanted devices represent an important complication in orthopaedic and dental surgery and may result in implant failure. Controlled release of antibacterial agents directly at the implant site may represent an effective approach to treat these chronic complications. Resistance to conventional antibiotics by pathogenic bacteria has emerged in recent years as a major problem of public health. In order to overcome this problem, non-conventional antimicrobial agents have been under investigation. In this study, polyacrylate-based hydrogel thin coatings have been electrosynthesised on titanium substrates starting from poly(ethylene glycol diacrylate)–co–acrylic acid. Silver nanoparticles (AgNPs) with a narrow size distribution have been synthesized using a “green” procedure and immobilized on Ti implant surfaces exploiting hydrogel coatings’ swelling capabilities. The coatings have been characterized by XPS and SEM/EDX, while their silver release performances have been monitored by ICP–MS. The antibacterial activity of these AgNP-modified hydrogel coatings was tested evaluating in vitro inhibition growth of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, among the most common pathogens in orthopaedic infections. Moreover, a preliminary investigation of the biocompatibility of silver-loaded coatings versus MG63 human osteoblast-like cells has been performed. An important point of strength of this paper, in fact, is the concern about the effect of silver species on the surrounding cell system in implanted medical devices. Silver ion release has been properly tuned in order to assure antibacterial activity while preserving osteoblasts’ response at the implant interface.

Silver nanoparticles-loaded PEGDA-AA hydrogel coatings for inhibition of titanium implants associated infections

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Duan K, Wang R (2006) J Mater Chem 16:2309–2321

    Article  CAS  Google Scholar 

  2. Zilberman M, Elsner JJ (2008) J Control Release 130:202–215

    Article  CAS  Google Scholar 

  3. Landini P, Antoniani D, Burgess JG, Nijland R (2010) Appl Microbiol Biotechnol 86(3):813–823

    Article  CAS  Google Scholar 

  4. Lentino JR (2003) Clin Infect Dis 36(9):1157–1161

    Article  Google Scholar 

  5. Høiby N, Ciofu O, Johansen HK, Song ZJ, Moser C, Jensen PØ, Molin S, Givskov M, Tolker-Nielsen T, Bjarnsholt T (2011) Int J Oral Sci 3(2):55–65

    Article  Google Scholar 

  6. Montanaro L, Speziale P, Campoccia D, Ravaioli S, Cangini I, Pietrocola G, Giannini S, Arciola CR (2011) Future Microbiol 6(11):1329–1349

    Article  CAS  Google Scholar 

  7. Arciola CR, An YH, Campoccia D, Donati ME, Montanaro L (2005) J Artific Organs 28(11):1091–1100

    CAS  Google Scholar 

  8. Carrega G, Bartolacci V, Burastero G, Casalino Finocchio G, Grappiolo G, Salomone C, Sandrone C, Santoriello L, Riccio G (2008) Infez Med 16(4):204–208

    CAS  Google Scholar 

  9. Zmistowski B, Fedorka CJ, Sheehan E, Deirmengian G, Austin MS, Parvizi J (2011) J Arthroplasty 26:104–108

    Article  Google Scholar 

  10. Cioffi N, Rai M (2012) Nano-antimicrobials. Progress and Prospects, Springer-Verlag Publisher, Eds, 1st Edition, 504 pp, ISBN 978-3-642-24427-8

  11. Rai M, Yadav A, Gade A (2009) Biotechnol Adv 27:76–83

    Article  CAS  Google Scholar 

  12. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA (2007) Burns 33:139–148

    Article  Google Scholar 

  13. Chaw KC, Manimaran M, Tay FEH (2005) Antimicrob Agents Chemother 49(12):4853–4859

    Article  CAS  Google Scholar 

  14. Alta V, Bechert T, Steinrucke P, Wagener M, Seidel P, Dingeldein E, Domann E, Schnettler R (2004) Biomaterials 25:4383–4391

    Article  Google Scholar 

  15. Croes S, Stobberingh EE, Stevens KNJ, Knetsch MLW, Koole LH (2011) Appl Mater Interfaces 3(7):2543–2550

    Article  CAS  Google Scholar 

  16. Varaprasad K, Murali MY, Vimala K, Mohana Raju K (2011) J Appl Polym Sci 121(2):784–796

    Article  CAS  Google Scholar 

  17. Sudheesh Kumar PT, Abhilash S, Manzoor K, Nair SV, Tamura H, Jayakumar R (2010) Carbohydr Polym 80:761–767

    Article  Google Scholar 

  18. Wen-Fu L, Kai-Tai T (2010) J Mater Sci 45(1):89–97

    Article  Google Scholar 

  19. Zan X, Kozlov M, McCarthy TJ, Su Z (2010) Biomacromolecules 11(4):1082–1088

    Article  CAS  Google Scholar 

  20. Tyllianakis M, Dalas E, Christofidou M, Kallitsis JK, Chrissanthopoulos A, Koutsoukos PG, Bartzavali C, Gourdoupi N, Papadimitriou K, Oikonomou EK (2010) J Mater Sci: Mater Med 21(7):2201–2211

    Article  CAS  Google Scholar 

  21. Das K, Bose S, Bandyopadhyay A, Karandikar B, Gibbins BL (2008) J Biomed Mater Res B: Appl Biomater 87B(2):455–458

    Article  CAS  Google Scholar 

  22. Ma Q, Mei S, Ji K, Zhang Y, Chu PK (2011) J Biomed Mater Res A 98A(2):274–286

    Article  CAS  Google Scholar 

  23. Liao J, Mo A, Zhu Z, Quan Y (2010) Int J Nanomedicine 5:337–342

    CAS  Google Scholar 

  24. Mahmood M, Li Z, Casciano D, Khodakovskaya MV, Chen T, Karmakar A, Dervishi E, Xu Y, Mustafa T, Watanabe F, Fejleh A, Whitlow M, Al-Adami M, Ghosh A, Biris AS (2011) J Cell Mol Med 15(11):2297–2306

    Article  CAS  Google Scholar 

  25. Saravanan S, Nethala S, Pattnaik S, Tripathi A, Moorthi A, Selvamurugan N (2011) Int J Biol Macromol 49(2):188–193

    Article  CAS  Google Scholar 

  26. Zhao L, Wang H, Huo K, Cui L, Zhang W, Ni H, Zhang Y, Wu Z, Chu PK (2011) Biomaterials 32(24):5706–5716

    Article  CAS  Google Scholar 

  27. Moaddab S, Ahari H, Shahbazzadeh D, Motallebi AA, Anvar AA, Rahman-Nya J, Shokrgozar MR (2011) Int Nano Lett 1(1):11–16

    CAS  Google Scholar 

  28. Xing ZC, Chae WP, Baek JY, Choi MJ, Jung Y, Kang IK (2010) Biomacromolecules 11(5):1248–1253

    Article  CAS  Google Scholar 

  29. Poliakoff M, Anastas P (2001) Nature 413:257–259

    Article  CAS  Google Scholar 

  30. Panigrahi S, Kundu S, Ghosh SK, Nath S, Pal T (2004) J Nanopart Res 6:411–414

    Article  CAS  Google Scholar 

  31. Raveendran P, Fu J, Wallen SL (2003) J Am Chem Soc 125:13940–13941

    Article  CAS  Google Scholar 

  32. Huang H, Yuan Q, Yang X (2004) Colloid Surf B 39:31–37

    Article  CAS  Google Scholar 

  33. Vigneshwaran N, Nachane RP, Balasubramanja RH, Varadarajan PV (2006) Carbohydr Res 341:2012–2018

    Article  CAS  Google Scholar 

  34. Singh M, Sinha I, Mandal RK (2009) Mater Lett 63:425–427

    Article  CAS  Google Scholar 

  35. De Giglio E, Cometa S, Ricci MA, Cafagna D, Savino AM, Sabbatini L, Orciani M, Ceci E, Novello L, Tantillo GM, Mattioli-Belmonte M (2011) Acta Biomater 7:882–891

    Article  Google Scholar 

  36. De Giglio E, Cometa S, Satriano C, Sabbatini L, Zambonin PG (2009) J Biomed Mater Res 88(4):1048–1057

    Article  Google Scholar 

  37. De Giglio E, Cafagna D, Ricci MA, Sabbatini L, Cometa S, Ferretti C (2010) J Bioact Compat Polym 25:374–391

    Article  Google Scholar 

  38. De Giglio E, Cometa S, Ricci MA, Zizzi A, Cafagna D, Manzotti S, Sabbatini L, Mattioli-Belmonte M (2010) Acta Biomater 6:282–290

    Article  Google Scholar 

  39. Solomon SD, Bahadory M, Jeyarajasingam AV, Rutkowsky SA, Boritz C (2007) J Chem Educ 84:322–325

    Article  CAS  Google Scholar 

  40. Kraemer EO, Stamm AJ (1924) J Am Chem Soc 46(12):2707–2709

    Article  Google Scholar 

  41. Scofield JH (1976) J Electron Spectrosc Relat Phenom 8(2):129–137

    Article  CAS  Google Scholar 

  42. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH (1985) J Clin Microbiol 22(6):996–1006

    CAS  Google Scholar 

  43. Liz Marzan LM, Lado-Tourino I (1996) Langmuir 12:3585–3589

    Article  CAS  Google Scholar 

  44. Esumi K, Tano T, Torigoe K, Meguro K (1990) Chem Mater 2:564–567

    Article  CAS  Google Scholar 

  45. Henglein A (2001) Langmuir 17:2329–2333

    Article  CAS  Google Scholar 

  46. Cioffi N, Ditaranto N, Torsi L (2005) Anal Bioanal Chem 382:1912–1918

    Article  CAS  Google Scholar 

  47. Lee PC, Meisel D (1982) J Phys Chem 86(17):3391–3395

    Article  CAS  Google Scholar 

  48. Creighton JA, Blatchford CG, Albrecht MG (1979) J Chem Soc Faraday Trans 2: Mol Chem Phys 75(5):790–798

    Article  CAS  Google Scholar 

  49. Guzmán MG, Dille J, Godet S (2009) Int J Chem Biomol Eng 2(3):104–111

    Google Scholar 

  50. Akbari B, Tavandashti MP, Zandrahimi M (2011) Iranian J Mater Sci Eng 8(2):48–56

    CAS  Google Scholar 

  51. Panăčk A, Kvítek L, Kolář M, Večeřová R, Pizúrová N, Sharma V, Nevĕčná T, Zbořil R (2006) J Phys Chem B 110:16248–16253

    Article  Google Scholar 

  52. Chairam S, Poolperm C, Somsook E (2009) Carbohyd Polym 75:694–704

    Article  CAS  Google Scholar 

  53. Monopoli A, Calò V, Ciminale F, Cotugno P, Angelici C, Cioffi N, Nacci A (2010) J Org Chem 75:3908–3911

    Article  CAS  Google Scholar 

  54. Trapani A, De Giglio E, Cafagna D, Denora N, Agrimi G, Cassano T, Gaetani S, Cuomo V, Trapani G (2011) Int J Pharm 419:296–307

    Article  CAS  Google Scholar 

  55. Russel AD, Hugo WB (1994) Prog Med Chem 31:351–370

    Article  Google Scholar 

  56. Lu L, Luo Y, JernNg W, Zhao XS (2009) Micropor Mesopor Mat 120:304–309

    Article  Google Scholar 

  57. Guzman M, Dille J, Godet S (2012) Nanomed-Nanotechnol 8:37–45

    Article  CAS  Google Scholar 

  58. Rai M, Yadav A, Gade A (2009) Biotechnol Adv 27:76–83

    Article  CAS  Google Scholar 

  59. Kittler S, Greulich C, Gebauer JS, Diendorf J, Treuel L, Ruiz L, Gonzalez-Calbet JM, Vallet-Regi M, Zellner R, Köller M, Epple M (2010) J Mater Chem 20:512–518

    Article  CAS  Google Scholar 

  60. Sayes CM, Liang F, Hudson JL, Mendez J, Guo W, Beach JM, Moore VC, Doyle CD, West JL, Billups WE, Ausmanb KD, Colvin VL (2006) Toxicol Lett 161:135–142

    Article  CAS  Google Scholar 

  61. Liu Z, Davis C, Cai W, He L, Chen X, Dai H (2008) Proc Natl Acad Sci 105:1410–1415

    Article  CAS  Google Scholar 

  62. Geiser M, Rothen-Rutishauser B, Kapp N, Schurch S, Kreyling W, Schulz H, Semmler M, ImHof V, Heyder J, Gehr P (2005) Environ Health Perspect 113:1555–1560

    Article  Google Scholar 

  63. Rothen-Rutishauser BM, Schurch S, Haenni B, Kapp N, Gehr P (2006) Environ Sci Technol 40:4353–4359

    Article  CAS  Google Scholar 

  64. Mahmood M, Casciano DA, Mocan T, Iancu C, Xu Y, Mocan L, Iancu DT, Dervishi E, Li Z, Abdalmuhsen M, Biris A, Ali N, Howard P, Biris AS (2010) J Appl Toxicol 30:74–83

    Article  CAS  Google Scholar 

  65. Travan A, Marsich E, Donati I, Benincasa M, Giazzon M, Felisari L, Paoletti S (2011) Acta Biomater 7(1):337–346

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to acknowledge Dott. Concetta Ferretti and Dott. Adriana Trapani for their technical support. The Laboratory “Laboratorio di tecnologie di modificazione superficiale di fibre naturali per il rilancio del settore tessile in Puglia–Avviso pubblico Reti di Laboratori Pubblici di Ricerca, Regione Puglia” and the research center “Laboratorio di Ricerca per la diagnostica dei Beni Culturali” (University of Bari Aldo Moro) are also acknowledged for providing access to TEM and ICP/MS facilities, respectively. This project was financed by the University of Bari Aldo Moro (Progetti d’Ateneo to Elvira De Giglio).

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70126, Bari, Italy

    E. De Giglio, D. Cafagna, A. Allegretta, A. Pedico, L. C. Giannossa & L. Sabbatini

  2. Department of Chemistry and Industrial Chemistry, University of Pisa, Via Risorgimento 35, 56126, Pisa, Italy

    S. Cometa

  3. Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Via Tronto 10/a, 60020, Ancona, Italy

    M. Mattioli-Belmonte

  4. Department of Biomedical Sciences and Human Oncology, University of Bari Aldo Moro, 70126, Bari, Italy

    R. Iatta

Authors
  1. E. De Giglio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Cafagna
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Cometa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Allegretta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Pedico
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. C. Giannossa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. L. Sabbatini
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Mattioli-Belmonte
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. R. Iatta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. De Giglio.

Additional information

Published in the special issue Analytical Science in Italy with guest editor Aldo Roda.

Rights and permissions

Reprints and permissions

About this article

Cite this article

De Giglio, E., Cafagna, D., Cometa, S. et al. An innovative, easily fabricated, silver nanoparticle-based titanium implant coating: development and analytical characterization. Anal Bioanal Chem 405, 805–816 (2013). https://doi.org/10.1007/s00216-012-6293-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-012-6293-z

Keywords

Search

Navigation