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Autoindicating optical properties of laccase as the base of an optical biosensor film for phenol determination

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Abstract

In the context of sustainable analytical chemistry, phenol has been determined through its enzymatic reaction with laccase. The method has been studied and optimized through the autoindicating optical properties of laccase both by intrinsic molecular absorption and fluorescence. The method shows a linear range from 9.79·10−6 to 7.50·10−4 M with a relative standard deviation of 1.07 %. The molecular absorption methodology has been implemented in a polyacrylamide film for the design of an autoindicating optical sensor. In order to increase the lifetime of the sensor, the reversibility study of the enzymatic reaction has proposed, as a novelty, the regeneration of laccase with an oxidase-type enzyme (glucose oxidase). The lifetime of the sensor film has improved from 15 to 30 measurements. The reaction mechanism has also been studied and confirmed by fluorescence and molecular absorption. The method leads to the determination of phenol in environmental samples.

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Acknowledgments

This work was supported by the Ministry of Science and Technology (MICINN) of Spain within the project CTQ2008-06751-C02-01/BQU and by the LaCaixa-DGA GA-LC-36/2011 project which are gratefully acknowledged.

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Authors and Affiliations

  1. Analytical Biosensors Group (GBA), Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, 50009, Zaragoza, Spain

    J. Sanz, S. de Marcos & J. Galbán

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  1. J. Sanz
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  2. S. de Marcos
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  3. J. Galbán
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Correspondence to S. de Marcos.

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Published in the special paper collection Progress on Environmental and Bioanalysis in Spain with guest editors Alfredo Sanz-Medel and Elena Domínguez.

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Sanz, J., de Marcos, S. & Galbán, J. Autoindicating optical properties of laccase as the base of an optical biosensor film for phenol determination. Anal Bioanal Chem 404, 351–359 (2012). https://doi.org/10.1007/s00216-012-6061-0

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  • DOI: https://doi.org/10.1007/s00216-012-6061-0

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