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A metallomics approach discovers selenium-containing proteins in selenium-enriched soybean

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Abstract

Our previous study found that high-molecular-weight selenium (Se) species make up 82% of the total Se in the bean of Se-enriched soybean plants (Chan et al. 2010, Metallomics, 2(2): p. 147–153). The Se species have been commonly seen in other plants in addition to soybean, but their identities remain unresolved. The present study employs a multi-technique metallomics approach to characterize the proteins containing Se in the beans of Se-enriched soybean plants. Two main categories of proteins, maturation proteins and protease inhibitors, were found in Se-containing high-performance liquid chromatography (HPLC) fractions. The proteins were screened by two-dimensional HPLC-inductively coupled plasma mass spectrometry, size-exclusion chromatography, and anion-exchange chromatography, and the Se-containing fractions were then identified by peptide mapping using HPLC-Chip-electrospray ion trap mass spectrometry. Based on the belief that Se goes into proteins through non-specific incorporation, a new method was designed and applied for the Se-containing peptide identification. The Se-containing peptide KSDQSSSYDDDEYSKPCCDLCMCTRS, part of the sequence of protein Bowman–Birk proteinase isoinhibitor (Glycine max), was found in one of the Se-containing fractions. The nutritional value of the Se-containing proteins in Se-enriched soybeans will be an interesting topic for the future studies.

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Acknowledgments

The authors are grateful to Agilent Technologies for instrument support and maintenance and to CEM for microwave instrumentation and support.

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  1. Department of Chemistry, University of Cincinnati, Cincinnati, OH, 45221-0172, USA

    Qilin Chan & Joseph A. Caruso

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  1. Qilin Chan
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  2. Joseph A. Caruso
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Correspondence to Joseph A. Caruso.

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Chan, Q., Caruso, J.A. A metallomics approach discovers selenium-containing proteins in selenium-enriched soybean. Anal Bioanal Chem 403, 1311–1321 (2012). https://doi.org/10.1007/s00216-012-5948-0

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