Abstract
Selenoproteins have been identified in a diverse range of organisms, including bacteria and animals. Their occurrence and role in the plant kingdom are, however, less well-understood. This work investigated the water-soluble selenium-containing proteins extracted from a selenium-accumulating plant species (Brassica juncea) and a nonaccumulator species (Helianthus annuus) exposed to varying forms and concentrations of selenium. Firstly, protein extracts were analyzed by size exclusion chromatography coupled to inductively coupled plasma mass spectrometry; specific detection was achieved by monitoring characteristic isotopes. Then, proteolytic digests of the plant extracts were analyzed by reversed phase chromatography coupled to ICP–MS in order to investigate selenoamino acid and selenopeptide content. Selenomethionine was observed to be the primary constituent of the proteins of the nonaccumulator plant, while selenocystine and selenomethionine were found in the same proportion in the accumulator extract. One main selenium-containing species was present at higher levels in the root digests than in the leaf digests; levels were greater in the nonaccumulator than in the accumulator plant.
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Acknowledgements
The research was partially supported by NIEHS-SBRP grant ES04908. The authors also acknowledge Agilent Technologies for their continuing support of our studies. We finally thank Mr. Austin Cupp for his assistance in sample preparation.
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Mounicou, S., Vonderheide, A.P., Shann, J.R. et al. Comparing a selenium accumulator plant (Brassica juncea) to a nonaccumulator plant (Helianthus annuus) to investigate selenium-containing proteins. Anal Bioanal Chem 386, 1367–1378 (2006). https://doi.org/10.1007/s00216-006-0707-8
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DOI: https://doi.org/10.1007/s00216-006-0707-8